Anti sqstm1 p62 5114

The primary antibodies of anti-LC3 (14600-1-AP) and anti-β-actin (60008-1-Ig), were purchased from Proteintech (Wuhan, Hubei, China). Anti-SQSTM1/p62 (5114) was purchased from Cell Signaling Technology (Danvers, Massachusetts, USA). The monoclonal antibody against the DTMUV E protein was prepared in our laboratory. Horseradish peroxidases (HRP) conjugated to goat anti-mouse secondary antibodies (BF03001) were purchased from Beijing Biodragon Immunotechnologies (Beijing, China). Rapamycin (Rapa) (HY-10219), 3-Methyladenine (3-MA) (HY-19312), chloroquine (CQ) (HY-17589), and were purchased from MedChemExpress (MCE, Monmouth Junction, New jersey, USA).

The primary antibodies of anti-LC3 (14600-1-AP), anti-Beclin 1 (11306-1-AP), anti-Flag (20543-1-AP), and anti-β-actin (60008-1-Ig) were purchased from Proteintech (Wuhan, China). Anti-SQSTM1/p62 (5114) was purchased from Cell Signaling Technology (Danvers, MA, USA) and anti-p-TBK1 (bs-3440R) was purchased from Bioss Antibodies (Beijing, China). The monoclonal antibody against E protein was custom-made by a biological company, and the immunogen was a recombinant E protein obtained from the DTMUV CQW1 E gene [20 (link)]. Horseradish peroxidases (HRP) conjugated to goat anti-rabbit (BF03008) or anti-mouse secondary antibodies (BF03001) were purchased from Beijing Biodragon Immunotechnologies (Beijing, China). Rapamycin (Rapa) (HY-10219), 3-methyladenine (3-MA) (HY-19312), chloroquine (CQ) (HY-17589), and aloxistatin (E64d) (HY-100229) were purchased from MedChemExpress (MCE, Monmouth Junction, NJ, USA).