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2 protocols using 3h 1 methyl 4 phenylpyridinium mpp

1

Radiolabeled Compound Uptake Assay

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[14C]Tetraethylammonium (TEA, 55 mCi/mmol) and [3H]1-methyl-4-phenylpyridinium (MPP+, 85 Ci/mmol) were obtained from American Radiolabeled Chemicals (St. Louis, MO, USA), [3H]atenolol (3.3 Ci/mmol) was from Moravek Biochemicals (Mercury Lane, Brea, CA, USA), and [3H]estrone-3-sulfate (54 Ci/mmol) was from PerkinElmer (Waltham, MA, USA). Unlabeled atenolol and Dulbecco’s modified Eagle’s medium (DMEM) were from Wako Pure Chemical Industries (Osaka, Japan). Fetal bovine serum (FBS) was obtained from Invitrogen (Carlsbad, CA, USA). All other regents were of analytical grade and commercially obtained.
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2

Radioligand Assay for Neurotransmitters

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Bulk chemicals were purchased from Sigma-Aldrich (Vienna, Austria). [3H] 1-methyl-4-phenylpyridinium (MPP+) was purchased from American Radiolabeled Chemicals (St. Louis, MO, United States) and non-tritiated MPP+ from Sigma-Aldrich (Vienna, Austria). Corticosterone, β-estradiol, and D22 were obtained from Sigma-Aldrich (Vienna, Austria), THP (Vienna, Austria) and Synthon Chemicals (Bitterfeld-Wolfen, Germany) respectively. Tetrodotoxin (TTX) was purchased from Latoxan (Valence, France).
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