Single cell suspensions were prepared from isolated lungs and stained for flow cytometry as described for nasal epithelial tissue [21 (link)]. Following fluorescent-labeled antibodies were used: APC/cy7*anti-rat CD45 (#202216, BD Biosciences), Brilliant Violet 421*anti-rat CD3 clone 1F4 (RV0) (#563948, BD Biosciences), APC*anti-rat CD11b clone WTS (RV0) (#562102 BD Biosciences), FITC* anti-rat RT1Dab (MHCII) (#205405, BioLegend, Inc.), PerCP/cy 5.5* anti-rat CD4 (#201519, BioLegend, Inc.), and PE* anti-rat CD161 (#205604, BioLegend Inc.). Live lymphocyte sized cells were stained with CD45 and CD3 to identify T cells. Further, the T cells were classified into T helper and cytotoxic T cells based on CD4 and CD8 expression, respectively. CD3-, CD11b-, CD4+ and MHCII+ cells were identified as plasmacytoid dendritic cells (pDCs). CD3- and CD161+ cells were identified as natural killer (NK) cells. Gating strategies have been elaborated in detail in our previous publication [21 (link)].
Apc cy7 anti rat cd45
Manufactured by BD
The APC/cy7*anti-rat CD45 is a fluorescently labeled antibody that binds to the CD45 antigen expressed on the surface of rat immune cells. It is a laboratory tool used for the identification and analysis of rat leukocyte populations by flow cytometry.
Automatically generated - may contain errors
Lab products found in correlation
Brilliant violet 421 anti rat cd3 clone 1f4 rv0, by BD (2 mentions)
Apc anti rat cd11b clone wts rv0, by BD (2 mentions)
Fitc anti rat rt1dab mhcii, by BioLegend (2 mentions)
Percp cy 5.5 anti rat cd4, by BioLegend (2 mentions)
Live dead fixable blue dead cell stain kit, by Thermo Fisher Scientific (1 mentions)
2 protocols using apc cy7 anti rat cd45
1
Multiparametric Flow Cytometry of Lung Immune Cells
2
Comprehensive Immune Cell Profiling
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Single cell suspensions were prepared and stained for flow cytometry during the same set of experiments described above. The following fluorescent-labeled antibodies were used: APC/cy7∗anti-rat CD45 (#202216, BD Biosciences), Brilliant Violet 421∗anti-rat CD3 clone 1F4 (RV0) (#563948, BD Biosciences), APC∗anti-rat CD11b clone WTS (RV0) (#562102 BD Biosciences), FITC∗ anti-rat RT1Dab (MHCII) (#205405, BioLegend, Inc.), PerCP/cy 5.5∗ anti-rat CD4 (#201519, BioLegends, Inc.), and PE∗ anti-rat CD161 (#205604, BioLegends Inc.). Dead cells were excluded by staining with LIVE/DEAD Fixable Blue Dead Cell stain kit (#L23105, ThermoFisher Scientific, Inc.) during analysis. Live lymphocyte sized cells were stained with CD45 and CD3 to identify T cells. Further the T cells were divided into T helper and cytotoxic T cells based on CD4 and CD8 expression, respectively. CD3-, CD11b-, CD4+ and MHCII + cells were identified as plasmacytoid dendritic cells (pDCs). CD3-and CD161 + cells were identified as natural killer (NK) cells (gating strategy is presented as Supplementary Material).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!