Non depc treated dnase rnase free water

All oligonucleotides were from Integrated DNA Technologies, Inc. (Coralville, IA). 2,2′-Azino-bis(3ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS), hemin, triton-X100 and HEPES were purchased from Sigma-Aldrich (St. Louis, MO). DMSO was from Fisher Scientific (Hampton, NH). Hydrogen peroxide solution (3%) was from VWR (Radnor, PA). Non-DEPC treated DNase/RNase-free water was from Boston Bio Products (Ashland, MA). GelRed® nucleic acid gel stain was from Biotium (Fremont, CA). All other reagents were of analytical grade.

Oligonucleotides were custom made by Integrated DNA Technologies, Inc. (Coralville, IA) and were used without purification, except the IPDz substrate, which was HPLC-purified. The NASBA liquid kit was purchased from Life Sciences Advanced Technologies, Inc. (Saint Petersburg, FL). 2,2’-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS), hemin, triton-X100 and HEPES were purchased from Sigma-Aldrich (St. Louis, MO). DMSO was from Fisher Scientific (Hampton, NH). Hydrogen peroxide solution (3%) was from VWR (Radnor, PA). Non-DEPC treated DNase/RNase-free water was from Boston Bio Products (Ashland, MA). SeaKem® LE agarose was from Lonza (Basel, Switzerland), GelRed® nucleic acid gel stain was from Biotium (Fremont, CA). RiboRuler Low Range RNA Ladder, Middlebrook 7H9 with OADC, and Ambion Turbo DNA-free DNase kit were obtained from ThermoFisher Scientific (Waltham, MA). All other reagents were of analytical grade. Absorption spectra were measured using NanoDrop OneC UV/Vis spectrometer (ThermoFisher Scientific, Waltham, MA). Amplification reactions were carried out using a C1000 Touch Thermo Cycler (Bio-Rad Laboratories, Inc., Hercules, CA). The amplicons were analyzed in 2% agarose gels supplemented with GelRed using a BioRad horizontal gel electrophoresis system. The gel images were acquired and analyzed using a BioRad GelDoc XR Molecular Imager.