BH3 profiling was performed by flow cytometry, as previously described.18 , 19 (link) Briefly, CLL cells were thawed from viably-frozen vials or harvested from ex vivo treatments, washed, and stained with fluorescent antibodies. Single cell suspensions were exposed to 0.002% digitonin and then BH3-only peptides for 60 minutes. After formaldehyde fixation, anti-cytochrome C-Alexa 488 (BD Pharmingen) was added, and to analyze the plate flow cytometry was performed.
Anti cytochrome c alexa 488
Manufactured by BD
Anti-cytochrome C-Alexa 488 is a fluorescently labeled antibody that binds to cytochrome C, a protein involved in cellular respiration. The Alexa 488 dye attached to the antibody allows for detection and visualization of cytochrome C in various biological applications.
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Lab products found in correlation
2 protocols using anti cytochrome c alexa 488
1
BH3 Profiling of CLL Cells by Flow Cytometry
2
BH3 Profiling of CLL Cells by Flow Cytometry
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