Bx50 microscope system

Manufactured by Olympus

Sourced in Japan

The BX50 microscope system is a high-performance optical microscope designed for a variety of laboratory applications. It features a sturdy, ergonomic design and a range of advanced optical components to provide clear, high-resolution images. The BX50 is capable of various magnification levels to accommodate different observation needs. The system is compatible with a wide range of accessories and can be customized to suit specific research or analysis requirements.

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BX50 microscope (556 citations) Olympus System Microscope (4 citations) BX50 System (2 citations)

5 protocols using bx50 microscope system

Histopathological Analysis of Rat Organ Tissues

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During
the experiment, animals
were sacrificed under ether anesthesia at specific time intervals
and following treatment, and important organs such as the lungs, liver,
spleen, and kidneys of rats from each group were rapidly removed.
The specimens were postfixed in buffered formalin (10% v/v) for 24
h. Tissues were sectioned (3–4 mm thick) after fixation, dried
with 100% alcohol, embedded in new paraffin, and allowed to cool.
Cross sections of each tissue were cut on a microtome and stained
with hematoxylin and eosin (H&E). Using an Olympus BX50 Microscope
System (Olympus, Japan), microphotographs were taken and the slides
were examined for histopathological anomalies.^{42 (link)}

Patel P., Raval M., Airao V., Ali N., Shazly G.A., Khan R, & Prajapati B. (2024). Formulation of Folate Receptor-Targeted Silibinin-Loaded Inhalable Chitosan Nanoparticles by the QbD Approach for Lung Cancer Targeted Delivery. ACS Omega, 9(9), 10353-10370.

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Histopathological Analysis of Tumor Specimens

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The tumor specimens were fixed in 10% buffered formalin solution for 12 h, followed by dehydration in 70%, 80%, 90%, 95%, and 100% ethanol for 2 h each time, then infiltrated with xylene and paraffin and embedded in paraplast. The specimens were serially sectioned (4 μm thickness) and stained with haematoxylin-eosin (H&E). The slides were observed for histopathological changes and microphotographs were taken using an Olympus BX50 microscope system (Olympus, Japan).

Liu S., Zheng L., Aweya J.J., Zheng Z., Zhong M., Chen J., Wang F, & Zhang Y. (2017). Litopenaeus vannamei hemocyanin exhibits antitumor activity in S180 mouse model in vivo. PLoS ONE, 12(8), e0183783.

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Histological Analysis of Brain and Pancreas Tissues

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For histological analyses, brain and pancreas tissue were kept in 10% buffered formalin solution. Brain and pancreatic tissues were cut into thin slices, dried, and then embedded in paraffin after being fixed in 10% buffered formalin solution. At least four cross-sections of 3–4 μm thickness were cut from each sample and subjected to staining with hematoxylin and eosin (H&E). Tissue sections were mounted with DPX mountant after two xylene washes that lasted two minutes each. After that, the slides were examined using bright-field microscopy. The Olympus BX50 microscope system (Olympus, Tokyo, Japan) was used for taking microphotographs.

Ahmad M.F., Naseem N., Rahman I., Imam N., Younus H., Pandey S.K, & Siddiqui W.A. (2022). Naringin Attenuates the Diabetic Neuropathy in STZ-Induced Type 2 Diabetic Wistar Rats. Life, 12(12), 2111.

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Quantifying Carcinoembryonic Antigen Expression

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Carcinoembryonic antigen expression areas were quantified using WinROOF Version 6.3 software (Mitani Corp., Tokyo, Japan) as previously described.28 Briefly, sections were observed and photographed using an Olympus BX50 microscope system (Olympus Corp., Tokyo, Japan). Five randomly selected fields of view (4.3 × 3.2 mm) were captured at 100× magnification, and the CEA expression area that stained brown was extracted automatically using two distinct macroinstructions composed chiefly of algorithms for color extraction based on red‐green‐blue (RGB) and hue‐luminosity‐saturation (HLS) parameters. CEA‐positive rate was determined by dividing the area stained with diaminobenzidine (DAB) by the entire area selected, and the average rate of the five fields was calculated.

Miyamoto Y., Muguruma N., Fujimoto S., Okada Y., Kida Y., Nakamura F., Tanaka K., Nakagawa T., Kitamura S., Okamoto K., Miyamoto H., Sato Y, & Takayama T. (2019). Epidermal growth factor receptor‐targeted molecular imaging of colorectal tumors: Detection and treatment evaluation of tumors in animal models. Cancer Science, 110(6), 1921-1930.

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Histological Analysis of Rat Liver

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For histological examination, hepatic sections of the liver were stained from different groups with hematoxylin and eosin (H & E). Brie y, at the end of the experiment, rats were anesthetized by the ether and transcardially perfused with saline. The liver was quickly removed and xed in formalin buffered (10%) for 24 hours. After xation is complete, slices (3-4 mm) of these tissues were dehydrated and embedded in para n. At least four cross sections of each tissue were taken with a thickness of 5 microns and stained with H & E. After two washing with xylene (2 min each), the tissue sections were tted with a DPX mountant. The slides were observed for pathological changes. Microscopic images were taken using the Olympus BX50 microscope system (Olympus, Japan) at King Khalid University Hospital, King Saud University, Saudi Arabia.

Abdelhalim M.A.K., AL-Mohy Y.H., & Al‐Ayed M.S. (2020). The Beneficial Use of Melanin in Inhibiting and Treating HCC Through Preventing CBC, Liver Enzymes, Oxidative Stress, and Lipid Peroxidation Alterations.

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