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Cc 4031g

Manufactured by Lonza
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Sourced in Germany

The CC-4031G is a laboratory centrifuge designed for general-purpose applications. It features a digital display, programmable speed and time settings, and an imbalance detection system. The centrifuge can accommodate a variety of sample tubes and microplates. Detailed specifications and intended use cases are not available.

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Lab products found in correlation

Cc 4021g, by Lonza (3 mentions) Cc4009g, by Lonza (2 mentions) Cc4017g, by Lonza (2 mentions) Cc4081g, by Lonza (2 mentions) Cc 3151, by Lonza (2 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions) Mcf 10a, by Lonza (1 mentions) Basic fgf, by Thermo Fisher Scientific (1 mentions) 24 well ultra low attachment plate, by Corning (1 mentions) Epidermal growth factor (egf), by Merck Group (1 mentions) Heparin, by Ratiopharm (1 mentions) Hydrocortisone, by Lonza (1 mentions) Insulin, by Lonza (1 mentions)

3 protocols using cc 4031g

1

Breast Cancer Cell Line Cultivation

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All the breast cell lines were obtained from the American Type Culture Collection (Manassas, VA, USA). The following cell lines were used: MCF‐10A cells, MCF‐7 cells, MDA‐MB‐468 cells, SK‐BR‐3 cells, MDA‐MB‐231 cells. MCF‐10A cells were cultured in Mammary Epithelial Cell Basal Medium (Lonza; CC3151) supplemented with 0.4% bovine pituitary extract (Lonza; CC4009G), 0.1% human epidermal growth factor (Lonza; CC4017G), 0.1% hydrocortisone (Lonza; CC4031G), 0.1% GA‐1000 (Lonza; CC4081G), and 0.1% insulin (Lonza; CC4021G). The other four cells were cultured in DMEM (Gibco‐BRL) with 10% fetal bovine serum (GIBCO‐BRL) and 1% penicillin/streptomycin (GIBCO‐BRL). All the cells were incubated in a humidified atmosphere of 5% CO2/95% air at 37 °C. Subcultivation of the cell lines was performed using 0.25% trypsin and 5 × 10−3m methylenediaminetetraacetic acid (EDTA) (Gibco‐BRL Co., MD, USA).
Ye S., Li W., Wang H., Zhu L., Wang C, & Yang Y. (2021). Quantitative Nanomechanical Analysis of Small Extracellular Vesicles for Tumor Malignancy Indication. Advanced Science, 8(18), 2100825.
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2

Sphere Assay Protocol for Cell Culture

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Sphere assays were conducted in MEBM medium (CC-3151, Lonza, Basel, Switzerland) supplemented with 4 μg/mL heparin (Ratiopharm, Ulm, Germany), 1x hydrocortisone (CC-4031G, Lonza), 1x insulin (CC-4021G, Lonza), 2% B-27 (#17504, Gibco, Life Technologies), 20 ng/ml EGF (E9644, Sigma), 20 ng/ml basic FGF (#100-18B, PeproTech, Rocky Hill, NJ, USA), and antibiotics. Unless indicated differently, 1250 cells were seeded into 300 μl medium and propagated in 24-well ultra-low attachment plates (#3473, Corning, NY, USA) at 37°C and 5% CO2. Sphere numbers were quantified at assay day 5 (i.e. after 120 hours of continuous incubation). Live single cells from trypsinized spheres were used for replating assays. For 3D-cultures, 5 × 105 cells were transferred to 10 ml sphere medium and propagated in 25-cm2 flasks (#3815, Corning) for 5 days, passaged by trypsinization, and analyzed after another 5 days of sphere cultivation.
Schaefer T., Wang H., Mir P., Konantz M., Pereboom T.C., Paczulla A.M., Merz B., Fehm T., Perner S., Rothfuss O.C., Kanz L., Schulze-Osthoff K, & Lengerke C. (2015). Molecular and functional interactions between AKT and SOX2 in breast carcinoma. Oncotarget, 6(41), 43540-43556.
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3

Cryopreservation and Culture of Pleural Effusion Cells

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Pleural effusions were collected from patients with metastatic breast cancer (mBC) or mesothelioma as waste materials during therapeutic drainage. Samples were anonymized by an honest broker and provided along with relevant clinical information, to the laboratory according to University of Pittsburgh IRB exemption (0503126, VSD). The cells were cryopreserved in 1.25mL vials (~50×106 cells/vial) in MEM containing 10% DMSO and 20% calf serum. The cryopreserved cells were carefully thawed in ice cold DMEM supplemented with 50% calf serum, suspended in complete mammary epithelial growth medium (MEGM, Catalog #CC-3151, Lonza, Walkersville, MD) supplemented with 10% fetal bovine serum (Catalog #SH30088.03, HyClone GE Healthcare Life Sciences) and 10% autologous cell-free pleural fluid. Complete MEGM consists of Epithelial Cell Basal Medium supplemented with bovine pituitary extract (BPE, Lonza Catalog #CC-4009G) human epidermal growth factor (rhEGF, 10ng/mL, Lonza Catalog #CC-4017G), hydrocortisone (0.5μg/mL, Lonza Catalog #CC-4031G), gentamicin sulfate plus amphotericin B (Lonza Catalog #CC-4081G) and insulin (10μg/mL, Lonza Catalog #CC-4021G). The cells were initially plated in 10 cm petri dishes at a culture density of 1 – 1.5 ×105 cells/cm2, grown to semi-confluence, trypsinized and split 1 to 3. All samples were analyzed at passage 0 and 1.
Singh M., Tian X.J., Donnenberg V.S., Watson A.M., Zhang J., Stabile L.P., Watkins S.C., Xing J, & Sant S. (2019). Targeting the temporal dynamics of hypoxia-induced tumor-secreted factors halts tumor migration. Cancer research, 79(11), 2962-2977.
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