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3 protocols using sodium salt of carboxymethylcellulose

1

Organogelator-Structured Oil Phase

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Monoglyceride Myverol™ (kindly provided by Kerry Bioscience, Bristol, United Kingdom) was used as an organogelator to structure the oil phase. The liquid paraffin, glycerol (≥99.5%), and sodium salt of carboxymethylcellulose (i.e., used as hydrogelator) were purchased from Sigma-Aldrich, Steinheim, Germany. The silica nanoparticles were also obtained from Sigma-Aldrich, Steinheim, Germany with an average diameter of 12 nm and specific surface area of 175–225 m2/g. Deionized water (DI) was obtained from a Milli-Q water purification system (Merck Millipore, Darmstadt, Germany). All the chemicals were of analytical grade and used as received without any purification.
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2

Chitosan and Chitin Preparation Protocol

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The chitosan (Mw ~ 370 kDa, DD 85%) and partially N-acetylated chitosan (Mw ~ 200 kDa, DD ~ 50%) were obtained from the Laboratory of Biopolymer Engineering of Institute of Bioengineering of Federal Research Center "Fundamentals of Biotechnology" of Russian Academy of Sciences (Moscow, Russia). Flake crab shell chitin was provided by BioProgress Co. (Shchelkovo, Russia) and grinded in laboratory mill. Colloidal chitin was prepared from powdered crab shell chitin by modi ed method of Rodriguez-Kabana et al. (1983) . Colloidal chitosan was prepared using the modi ed procedure (Helistö et al. 2001 (link)). N-acetyl-β-D-glucosamine, D-glucosamine, p-nitrophenyl-N-acetyl-β-D-glucosaminide (p-NP-GlcNAc), p-nitrophenyl-N,N'-diacetyl-β-D-chitobioside (p-NP-GlcNAc 2 ), p-nitrophenol, sodium salt of carboxymethylcellulose, azokazein, laminarin and xylan from birch were purchased from Sigma Chemical Co., USA. CM-Sepharose Fast Flow was purchased from GE Healthcare (USA). The nutritional components of culture media (tryptone, yeast extract etc.) were obtained from Panreac (Barcelona, Spain) and HiMedia Laboratories Pvt. Ltd. (Mumbai, India). All other reagents, including components for SDS-PAGE, TLC, buffer salts etc. used in the study were quali ed as analytical and high grade.
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3

Chitosan and Chitin Preparation Protocol

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The chitosan (Mw ~ 370 kDa, DD 85%) and partially N-acetylated chitosan (Mw ~ 200 kDa, DD ~ 50%) were obtained from the Laboratory of Biopolymer Engineering of Institute of Bioengineering of Federal Research Center "Fundamentals of Biotechnology" of Russian Academy of Sciences (Moscow, Russia). Flake crab shell chitin was provided by BioProgress Co. (Shchelkovo, Russia) and grinded in laboratory mill. Colloidal chitin was prepared from powdered crab shell chitin by modi ed method of Rodriguez-Kabana et al. (1983) . Colloidal chitosan was prepared using the modi ed procedure (Helistö et al. 2001 (link)). N-acetyl-β-D-glucosamine, D-glucosamine, p-nitrophenyl-N-acetyl-β-D-glucosaminide (p-NP-GlcNAc), p-nitrophenyl-N,N'-diacetyl-β-D-chitobioside (p-NP-GlcNAc 2 ), p-nitrophenol, sodium salt of carboxymethylcellulose, azokazein, laminarin and xylan from birch were purchased from Sigma Chemical Co., USA. CM-Sepharose Fast Flow was purchased from GE Healthcare (USA). The nutritional components of culture media (tryptone, yeast extract etc.) were obtained from Panreac (Barcelona, Spain) and HiMedia Laboratories Pvt. Ltd. (Mumbai, India). All other reagents, including components for SDS-PAGE, TLC, buffer salts etc. used in the study were quali ed as analytical and high grade.
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