Several pesticides and metabolites (more than 130 analytes) were monitored in the current study by applying liquid and gas chromatography–tandem mass spectrometry (LC-MS/MS, GC-MS/MS). Analytical standards and materials used are described in previous works of our group [11 (link),15 (link),16 (link),17 (link)]. Carbendazim-d3, imidacloprid-d4, dimethoate-d6, and chlorpyrifos-d10 were used as mass-labeled internal standards for the quantification of the compounds measured by LC-MS/MS, and they were purchased from Sigma-Aldrich (Seelze, Germany). Triphenyl phosphate (TPP), deltamethrin-d6, and dichlorvos-d6 were used in the GC-MS/MS analysis and acquired from Sigma-Aldrich (Seelze, Germany), respectively.
Chlorpyrifos d10
Manufactured by Merck Group
Sourced in Germany
Chlorpyrifos-d10 is a deuterium-labeled organic compound used as an analytical standard in various laboratory applications. It is a stable isotope version of the insecticide chlorpyrifos, where 10 hydrogen atoms have been replaced with deuterium. This labeled compound is commonly used in analytical techniques, such as mass spectrometry, to aid in the identification and quantification of chlorpyrifos in environmental and biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Carbendazim d3, by Merck Group (2 mentions)
Dimethoate d6, by Merck Group (2 mentions)
Imidacloprid d4, by Merck Group (2 mentions)
Sulfuric acid, by Thermo Fisher Scientific (1 mentions)
Ammonium formate, by Thermo Fisher Scientific (1 mentions)
Acetonitrile, by Thermo Fisher Scientific (1 mentions)
Formic acid, by Thermo Fisher Scientific (1 mentions)
Methanol, by Thermo Fisher Scientific (1 mentions)
Dichloromethane dcm, by Merck Group (1 mentions)
Methanol, by Merck Group (1 mentions)
Acetonitrile, by Merck Group (1 mentions)
Sulphuric acid, by Scharlab (1 mentions)
Analytical standards, by Merck Group (1 mentions)
Chloroform, by Erba Group (1 mentions)
Ethyl acetate, by Avantor (1 mentions)
Formic acid, by Merck Group (1 mentions)
4 protocols using chlorpyrifos d10
1
Pesticides and Metabolites Monitoring
2
Quantitative Analysis of Pesticide Residues
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Certified chemical standards were obtained from Dr. Ehrenstorfer (Augsburg, Germany) and Sigma Aldrich (Seelze, Germany). Mass-labelled internal standards (imidacloprid-d4, chlorpyrifos d-10, carbendazim-d3, and dimethoate d-6) used for the quantification of the target analytes in the LC-ESI-MS/MS method were obtained from Sigma Aldrich (Seelze, Germany).
Standard solutions were prepared in acetonitrile and methanol in volumetric flasks and kept at -18°C away from the light. acetonitrile, methanol, ammonium formate, and formic acid of LC-MS grade were obtained from Fisher Scientific (Hampton, NH, USA). Dichloromethane (DCM) was of pesticide residues grade and was obtained from Sigma Aldrich (Seelze, Germany). Sodium sulfate anhydrous (Na 2 SO 4 ), and sulfuric acid (purity >95%) were obtained from Fisher Scientific. Hybrid SPE-phospholipid cartridges (30 mg, 1 mL) were purchased from Supelco (Bellefonte, PA, USA). All reagents and chemicals were of analytical grade.
Standard solutions were prepared in acetonitrile and methanol in volumetric flasks and kept at -18°C away from the light. acetonitrile, methanol, ammonium formate, and formic acid of LC-MS grade were obtained from Fisher Scientific (Hampton, NH, USA). Dichloromethane (DCM) was of pesticide residues grade and was obtained from Sigma Aldrich (Seelze, Germany). Sodium sulfate anhydrous (Na 2 SO 4 ), and sulfuric acid (purity >95%) were obtained from Fisher Scientific. Hybrid SPE-phospholipid cartridges (30 mg, 1 mL) were purchased from Supelco (Bellefonte, PA, USA). All reagents and chemicals were of analytical grade.
3
Hydrophobic Pesticides Extraction and Analysis
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The pesticides studied include three hydrophobic (log Kow > 4) compounds: CHL
Stock solutions of each pesticide in methanol were prepared using analytical standards purchased from Sigma-Aldrich (Steinheim, Germany) and stored in the dark at -20 °C until use.
Internal standards of d10-chlorpyrifos and phenoxy-d5-fenvalerate were also purchased from Sigma-Aldrich; d7-oxadiazon was purchased from LGC standards (Teddington, Middlesex, UK).
Ethyl acetate, acetone, water, dichloromethane, hexane, and methanol solvents were purchased form J.T. Baker (Waltham, Massachusetts, USA). Chloroform was purchased from Carlo Erba (Val De Reuil, Eure, France). Formic acid (98-100%) was purchased from Merck (Darmstadt, Germany). The quality of all solvents was according to organic trace analysis. Sulphuric acid was obtained from Scharlau (Spain), and mineral salts and other chemicals were purchased from Scharlab (Spain). GF/A and CG/C glass fibre filters were obtained from Whatman (GE Healthcare, USA). Table 1 (a) [32] , (b) [33] , (c) [34] (link), (d) [35] (link), (e) [36] (link)
Stock solutions of each pesticide in methanol were prepared using analytical standards purchased from Sigma-Aldrich (Steinheim, Germany) and stored in the dark at -20 °C until use.
Internal standards of d10-chlorpyrifos and phenoxy-d5-fenvalerate were also purchased from Sigma-Aldrich; d7-oxadiazon was purchased from LGC standards (Teddington, Middlesex, UK).
Ethyl acetate, acetone, water, dichloromethane, hexane, and methanol solvents were purchased form J.T. Baker (Waltham, Massachusetts, USA). Chloroform was purchased from Carlo Erba (Val De Reuil, Eure, France). Formic acid (98-100%) was purchased from Merck (Darmstadt, Germany). The quality of all solvents was according to organic trace analysis. Sulphuric acid was obtained from Scharlau (Spain), and mineral salts and other chemicals were purchased from Scharlab (Spain). GF/A and CG/C glass fibre filters were obtained from Whatman (GE Healthcare, USA). Table 1 (a) [32] , (b) [33] , (c) [34] (link), (d) [35] (link), (e) [36] (link)
4
Analytical Workflow for Environmental Pollutants
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The following materials and chemicals were purchased from Thermo Fisher Scientific Labeled standards d14-trifluralin, d6-alpha-HCH, d8-p,p'-DDT and d4-alpha-endosulfan were obtained from CDN Isotope Inc. (Quebec, Canada). d10-chlorpyrifos was purchased from Sigma-Aldrich, Inc. (Missouri, USA).
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