Log In Sign up
The largest database of trusted experimental protocols

Prrlsin cppt pgk

Manufactured by Addgene
Visit Supplier

PRRLSIN.cPPT.PGK is a plasmid that contains a self-inactivating lentiviral vector system. It provides the core components necessary for lentiviral production and transduction.

Automatically generated - may contain errors

Lab products found in correlation

Amicon ultra 15 100k, by Merck Group (1 mentions) Pwpt gfp, by Addgene (1 mentions)

Close spelling variants of this product

PRRLSIN.cPPT.PGK-GFP.WPRE (8 citations) PRRLSIN.cPPT.PGK-GFP.WPRE lentiviral vector (3 citations) PRRLSIN.cPPT.PGK vector (1 citations)

2 protocols using prrlsin cppt pgk

1

Lentiviral Transduction of PR Variants

Check if the same lab product or an alternative is used in the 5 most similar protocols
Coding sequences for PR_A, PR_Ai, PR_A2mut, PR_Ai2mut, PR_A3mut, and PR_Ai3mut were recloned into the lentiviral vector pRRLSIN.cPPT.PGK (Addgene plasmid #12252; a gift from Dr Trono) generating pLVPR_A, pLVPR_Ai, pLVPR_A2mut, pLVPR_Ai2mut, pLVPR_A3mut, and pLVPR_Ai3mut, respectively. Lentiviral particles were produced by the transient transfection of HEK293T cells by Eurogen (Moscow, Russia) as described elsewhere [42 (link)] and concentrated 10-fold with Amicon Ultra-15 100K centrifuge concentrators (Merck-Millipore, Darmstadt, Germany). Infectious viral particles were formed only by pLVPR_A3i; their titer was determined on HT1080 cells with quantitative real-time PCR [42 (link)] using standard samples of HT-1080 DNA with a known number of viral genome copies.
Petkov S., Kilpeläinen A., Bayurova E., Latanova A., Mezale D., Fridrihsone I., Starodubova E., Jansons J., Dudorova A., Gordeychuk I., Wahren B, & Isaguliants M. (2022). HIV-1 Protease as DNA Immunogen against Drug Resistance in HIV-1 Infection: DNA Immunization with Drug Resistant HIV-1 Protease Protects Mice from Challenge with Protease-Expressing Cells. Cancers, 15(1), 238.
+ Open protocol
+ Expand
2

Lentiviral Production of ChR2-EYFP

Check if the same lab product or an alternative is used in the 5 most similar protocols
To generate a third generation ChR2-EYFP expressing lentivirus ( pRRL-Ef1a-ChR2-EYFP), a ChR2-EYFP fragment from the pcDNA3.1/hChR2(H134R)-EYFP vector (kindly provided by K. Deisseroth) and an EF1α promoter fragment from pWPT-GFP (Addgene 12255) were cloned into the backbone pRRLSIN.cPPT.PGK (Addgene 12252, both kindly provided by D. Trono through Addgene). Lentiviral particles were prepared and as previously reported; 14 they were kindly provided by K. Zimmermann and A. Pfeifer, Department of Pharmacology and Toxicology, University of Bonn.
Agladze N.N., Halaidych O.V., Tsvelaya V.A., Bruegmann T., Kilgus C., Sasse P, & Agladze K.I. (2017). Synchronization of excitable cardiac cultures of different origin. Biomaterials science, 5(9).
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!