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3 6 di o thiosalicyl fluorescein ssp4

Manufactured by Dojindo Laboratories
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Sourced in Japan

3',6'-di(O-thiosalicyl)fluorescein (SSP4) is a fluorescent dye compound developed by Dojindo Laboratories. It is a derivative of fluorescein with two thiosalicyl substituents. The core function of SSP4 is to act as a fluorescent probe for various analytical and research applications.

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Lab products found in correlation

Monobromobimane, by Merck Group (2 mentions) Cumene hydroperoxide, by Merck Group (2 mentions) Erastin, by Merck Group (2 mentions) Liproxstatin 1, by Merck Group (2 mentions) Disodium sulfide, by Merck Group (2 mentions) Diallyl tetrasulfide, by Thermo Fisher Scientific (2 mentions) 4 hydroxy 2 2 6 6 tetramethylpiperidin 1 oxyl tempol, by Merck Group (2 mentions) 7 diethylamino coumarin 3 carbohydrazide, by Merck Group (2 mentions) C11 bodipy, by Thermo Fisher Scientific (2 mentions) 1 p hydroxyphenyl imidazole, by Merck Group (2 mentions) Depmpo, by Enzo Life Sciences (2 mentions) 3 mercaptopyruvate, by Merck Group (2 mentions) 5 aminolevulinic acid hydrochloride, by Merck Group (2 mentions) 2 2 azino bis 3 ethylbenzothiazoline 6 sulfonic acid, by Merck Group (2 mentions) Amplex red, by Thermo Fisher Scientific (2 mentions) S nitrosoglutathione, by Merck Group (2 mentions) 4 hydroxytamoxifen, by Merck Group (2 mentions) Cysteine, by Merck Group (2 mentions) Ferrocene, by Merck Group (2 mentions) Glutathione (gsh), by Merck Group (2 mentions)

3 protocols using 3 6 di o thiosalicyl fluorescein ssp4

1

Synthesis and Analysis of Sulfur Compounds

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3-aminophthalhydrazide (luminol), 1-(p-hydroxyphenyl)imidazole (HPI), disodium sulfide (Na2S), erastin, RSL3, cumene hydroperoxide (CHP), liproxstatin-1, 7-(diethylamino)coumarin-3-carbohydrazide (CHH), 4-hydroxytamoxifen (Tam), monobromobimane (MBB), cysteine (Cys), 3-mercaptopyruvate (3MP), cytochrome c (cyt c), glutathione reductase (GR), 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), S-nitrosoglutathione (GSNO), ferrocene, 4-hydroxy-2,2,6,6-tetramethylpiperidin-1-oxyl (TEMPOL) and isopropyl β-d-1-thiogalactopyranoside (IPTG) were obtained from Sigma-Aldrich. BODIPY C11, diallyl tetrasulfide (DATS) and Amplex Red were obtained from Thermo Fisher Scientific. DEPMPO was obtained from Enzo. H2O2 was obtained from Roth. GSH and 5-aminolevulinic acid hydrochloride were obtained from Merck. Sodium disulfide (Na2S2), sodium tetrasulfide (Na2S4) and 3′,6′-di(O-thiosalicyl)fluorescein (SSP4) were obtained from Dojindo. The synthesis of GSSSG, GS34SSG and GSSSSG was performed as described previously43 . CSSSC was synthesized as described below.
Barayeu U., Schilling D., Eid M., Xavier da Silva T.N., Schlicker L., Mitreska N., Zapp C., Gräter F., Miller A.K., Kappl R., Schulze A., Friedmann Angeli J.P, & Dick T.P. (2022). Hydropersulfides inhibit lipid peroxidation and ferroptosis by scavenging radicals. Nature Chemical Biology, 19(1), 28-37.
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2

Quantification of Sulfur Compounds

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All-trans retinoic acid (atRA), methylglyoxal 1, and 1-dimethyl acetal (MGDA) were purchased from Sigma (St. Louis, MO, USA). Dulbecco's modified Eagle's medium (DMEM)/Ham's F-12 (F12) medium was obtained from Life Technologies, Inc. (Grand Island, NY). A protease inhibitor, phosphatase inhibitor, and DMEM/F12 medium without cystine (Cys-free) were purchased from Nacalai Tesque (Kyoto, Japan). Na2S·9H2O and formic acid were from Wako (Osaka, Japan). Na2S3, 4-fluoro-7-sulfamoylbenzofurazan (ABD-F), 1,2-diamino-4,5-methylenedioxybenzene, dihydrochloride (DMB), and 3′,6′-di(O-thiosalicyl)fluorescein (SSP4) were obtained from Dojindo (Kumamoto, Japan). Monobromobimane (mBB) was from Calbiochem (La Jolla, CA, USA). RIPA lysis buffer was purchased from Millipore (Billerica, MA, USA).
Koike S., Nishimoto S, & Ogasawara Y. (2017). Cysteine persulfides and polysulfides produced by exchange reactions with H2S protect SH-SY5Y cells from methylglyoxal-induced toxicity through Nrf2 activation. Redox Biology, 12, 530-539.
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3

Redox Signaling Compound Synthesis and Detection

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3-Aminophthalhydrazide (luminol), 1-(p-hydroxyphenyl)imidazole (HPI), disodium sulfide (Na2S), erastin, RSL3, cumene hydroperoxide (CHP), liproxstatin-1, 7- (Diethylamino)coumarin-3-carbohydrazide (CHH), 4-hydroxytamoxifen (Tam), monobromobimane (MBB), cysteine (Cys), 3-mercaptopyruvate (3-MP), cytochrome c (cyt c), glutathione reductase (GR), 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS), S- nitrosoglutathione (GSNO), ferrocene, 4-hydroxy-2,2,6,6-tetramethylpiperidin-1-oxyl (TEMPOL) and isopropyl β-D-1-thiogalactopyranoside (IPTG) were obtained from Sigma. BODIPY C11, diallyl tetrasulfide (DATS) and Amplex Red was obtained from ThermoFisher. DEPMPO was obtained from Enzo. H2O2 was obtained from Roth. Glutathione (GSH) and 5- aminolevulinic acid hydrochloride was obtained from Merck. Sodium disulfide (Na2S2), sodium tetrasulfide (Na2S4), and 3',6'-Di(O-thiosalicyl)fluorescein (SSP4) were obtained from Dojindo. The synthesis of GSSSG, GS34SSG and GSSSSG was performed as described previously 43 . CSSSC was synthesized as described below.
Barayeu U., Schilling D., Eid M., Xavier da Silva T.N., Schlicker L., Mitreska N., Zapp C., Gräter F., Miller A.K., Kappl R., Schulze A., Friedmann Angeli J.P, & Dick T.P. (2022). Hydropersulfides inhibit lipid peroxidation and ferroptosis by scavenging radicals. Nature Chemical Biology, 19(1), 28-37.
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