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Pure link mini rna extraction kit

Manufactured by Thermo Fisher Scientific
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Sourced in United States

The Pure Link Mini RNA extraction kit is a laboratory equipment product designed for the rapid and efficient extraction of RNA from a variety of sample types. The kit utilizes a silica-based membrane technology to capture and purify RNA, allowing for high-quality RNA isolation.

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Lab products found in correlation

Itaq universal probes mastermix, by Bio-Rad (2 mentions) Taqman primer gene expression assays, by Thermo Fisher Scientific (2 mentions) Nanodrop spectrophotometer, by Thermo Fisher Scientific (2 mentions)

Close spelling variants of this product

RNA pure link mini kit Pure Link RNA RNA Mini Kit RNA extraction kit

2 protocols using pure link mini rna extraction kit

1

RNA Isolation, cDNA Synthesis, and RT-qPCR Analysis

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mRNA was isolated from up to 100mg of tissue and homogenized in 1mL of lysis buffer reagent provided in the Pure Link Mini RNA extraction kit (Ambion, Austin, TX) as previously described (Boesen et al., 2008 (link)). Following isolation, mRNA concentration was determined using a Nanodrop spectrophotometer (Model 2000, Thermo Scientific, Wilmington, Delaware). cDNA was made using the Biorad cDNA synthesis kit using 1ug of RNA. Finally, real time PCR was carried out using iTaq Universal Probes Mastermix (BioRad) and Taqman primer gene expression assays (Applied Biosystems). Gene expression was quantified using 2ΔΔCT method.
Heimlich J.B., Speed J.S., Bloom C.J., O'Connor P.M., Pollock J.S, & Pollock D.M. (2014). ET-1 increases reactive oxygen species following hypoxia and high salt diet in the mouse glomerulus. Acta physiologica (Oxford, England), 213(3), 722-730.
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2

Gene Expression Analysis of Oxidative Stress Markers

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mRNA was isolated using the Pure Link Mini RNA extraction kit (Ambion, Austin, TX) (Boesen, et al 2008 (link)) Real time polymerase chain reaction was carried out using iTaq Universal Probes Mastermix (Biorad, Hercules, CA, USA) and Taqman primer gene expression assays (Applied Biosystems, Foster City, CA, USA; CYBB (NOX2): Mm01287743_m1, NOX4: Mm00479246_m1, EDN1 (ET-1): Mm00438656_m1; GAPDH: Mm999999_g1). Results were quantified using 2-ΔΔCt and GAPDH served as the internal control (Schmittgen and Livak 2008 (link)).
Zahr R.S., Chappa P., Yin H., Brown L.A., Ataga K.I, & Archer D.R. (2018). Renal Protection by Atorvastatin in a Murine Model of Sickle Cell Nephropathy. British journal of haematology, 181(1), 111-121.
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