Gentamicin sulfate

Two human ovarian cancer cell lines were used, A2780 and SKOV-3. A2780 was purchased from European Collection of Authenticated Cell Cultures (ECACC®, 93112519) and were cultured in RPMI-1640 medium with 2 mM l-glutamine, 10% (v/v) fetal bovine serum (FBS, Biological Industries) and 0.1% (v/v) gentamicin (50 mg/ml gentamicin sulfate, Biological Industries), in a humidified atmosphere containing 5% CO 2 , at 37 °C. SKOV-3 were purchased from American Type Culture Collection (ATCC® HTB-77™) and were cultured in Dulbecco's modified Eagle's medium (DMEM, Gibco) supplemented with 10% (v/v) fetal bovine serum (Biological Industries), and 0.1% (v/v) gentamicin (50 mg/ml gentamicin sulfate, Biological Industries), in a humidified atmosphere containing 5% CO 2 , at 37 °C. For the adhesion assay, dermal endothelium cells were used. HMEC-1, human microvascular endothelial cells-purchased from American Type Culture Collection (ATCC®, CRL-3243™) were cultured in MCDB131 (Gibco) supplemented with 10 ng/ml Epidermal Growth Factor (EGF, Thermo Fischer Scientific), 1 µg/ml hydrocortisone (Sigma-Aldrich), 10 mM glutamine (Sigma-Aldrich), 10% (v/v) fetal bovine serum (Biological Industries), and 0.1% (v/v) gentamicin (50 mg/ ml gentamicin sulfate, Biological Industries), in a humidified atmosphere containing 5% CO 2 , at 37 °C.

Human ovarian carcinoma cell line (A2780, European Collection of Cell Culture, ECACC® 93112519) used in the study was established from tumor tissue of untreated patient. Human breast cancer cell line (MCF7, American Type Culture Collection, ATCC® HTB-22™) used in the study was derived from pleural effusion as a metastatic site.
Cells were cultured in RPMI-1640 medium (Biowest) including L-glutamine, supplemented with 10% (v/v) fetal calf serum (Biological Industries) and 0.1% (v/v) gentamicin (50 mg/mL gentamicin sulfate, Biological Industries) in a humidified atmosphere containing 5% CO 2 at 37°C.