Log In Sign up
The largest database of trusted experimental protocols

Ultrathin carbon type a 400 mesh copper grid

Manufactured by Ted Pella
Visit Supplier
Sourced in United States

Ultrathin carbon type-A 400 mesh copper grids are a type of laboratory equipment used for sample preparation in electron microscopy. They provide a support structure for thin samples, enabling high-resolution imaging and analysis.

Automatically generated - may contain errors

Lab products found in correlation

Tecnai 12 tem, by Thermo Fisher Scientific (2 mentions) Cell culture water, by Lonza (1 mentions) Tecnai g2, by Thermo Fisher Scientific (1 mentions) Tecnai g2 f20 x twin transmission electron microscope, by Thermo Fisher Scientific (1 mentions)

5 protocols using ultrathin carbon type a 400 mesh copper grid

1

Negative Staining of PA Micelles

Check if the same lab product or an alternative is used in the 5 most similar protocols
Ultrathin carbon type-A 400 mesh copper grids (Ted Pella, Redding, CA) were loaded with 5 μL of 0.5 mM PA micelles and allowed to dry. Grids were washed with several drops of water and then negatively stained with 1% aqueous phosphotungstic acid for 3 min. The excess solution was then removed, and grids were left to dry. Grids were imaged on a FEI Tecnai 12 TEM using an accelerating voltage of 120 kV.
Acar H., Samaeekia R., Schnorenberg M.R., Sasmal D.K., Huang J., Tirrell M.V, & LaBelle J.L. (2017). Cathepsin-Mediated Cleavage of Peptides from Peptide Amphiphiles Leads to Enhanced Intracellular Peptide Accumulation. Bioconjugate chemistry, 28(9), 2316-2326.
+ Open protocol
+ Expand
2

Negative Staining of BIM PAs

Check if the same lab product or an alternative is used in the 5 most similar protocols
Ultrathin carbon type-A 400 mesh copper grids (Ted Pella, Redding, CA, USA) were loaded with 1 μL of BIM PAs and allowed to dry. The grids were washed with several drops of water and then negatively stained with 1% aqueous phosphotungstic acid for 1 min. The excess solution was then removed and grids were left to dry. All the grids were imaged on a FEI Tecnai 12 TEM (Hillsboro, OR, USA) using an accelerating voltage of 120 kV.
Schnorenberg M.R., Bellairs J.A., Samaeekia R., Acar H., Tirrell M.V, & LaBelle J.L. (2019). Activating the Intrinsic Pathway of Apoptosis Using BIM BH3 Peptides Delivered by Peptide Amphiphiles with Endosomal Release. Materials, 12(16), 2567.
+ Open protocol
+ Expand
3

Characterizing Nanoceria Particles via TEM

Check if the same lab product or an alternative is used in the 5 most similar protocols
Micrographs were taken with a Tecnai 12 TEM operating at 80 kV equipped with a 1K×1K Keen View camera. Nanoceria dispersions were deposited on ultrathin carbon type-A 400 mesh copper grids (Ted Pella, Inc.). Micrographs were analyzed using ImageJ software for 200 particles.
Goujon G., Baldim V., Roques C., Bia N., Seguin J., Palmier B., Graillot A., Loubat C., Mignet N., Margaill I., Berret J.F, & Beray-Berthat V. (2021). Antioxidant Activity and Toxicity Study of Cerium Oxide Nanoparticles Stabilized with Innovative Functional Copolymers. Advanced healthcare materials, 10(11).
+ Open protocol
+ Expand
4

Visualizing Cry3Aa Protein Crystals by TEM

Check if the same lab product or an alternative is used in the 5 most similar protocols
Transmission electron microscopy (TEM) imaging was performed at the OSU Campus Microscopy and Imaging Facility (OSU CMIF). Samples were prepared by dispersing 0.5 mg Cry3Aa and Cry3Aa-GFP crystals in 1 mL cell culture water (Lonza). The samples were sonicated for 5 min and were stained with 6 drops of 3% phosphotungstic acid. The carbon grids were prepared by slowly dipping an Ultrathin Carbon Type-A 400 Mesh Copper Grid (Ted Pella) into solutions of either the Cry3Aa or Cry3Aa-GFP protein crystal three times and drying the grid at ambient temperature. A FEI Tecnai G2 transmission electron microscope operating at 200 kV was used to obtain TEM micrographs of the crystals.
Nair M.S., Lee M.M., Bonnegarde-Bernard A., Wallace J.A., Dean D.H., Ostrowski M.C., Burry R.W., Boyaka P.N, & Chan M.K. (2015). Cry Protein Crystals: A Novel Platform for Protein Delivery. PLoS ONE, 10(6), e0127669.
+ Open protocol
+ Expand
5

TEM Imaging of Dilute Samples

Check if the same lab product or an alternative is used in the 5 most similar protocols
Transmission electron microscopy images were taken on a FEI Tecnai G2 F20 X-TWIN Transmission Electron Microscope operated at 200 kV. Samples were prepared by dropcasting approximately 10 μL of a dilute solution of the sample in hexanes onto a ultrathin carbon type-A, 400 mesh, copper grid from Ted Pella, Inc.
Siefe C., Mehlenbacher R.D., Peng C.S., Zhang Y., Fischer S., Lay A., McLellan C.A., Alivisatos A.P., Chu S, & Dionne J.A. (2019). Sub-20 nm Core–Shell–Shell Nanoparticles for Bright Upconversion and Enhanced Förster Resonant Energy Transfer. Journal of the American Chemical Society, 141(42), 16997-17005.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!