DMEM (high glucose) and horse serum were from Sigma‐Aldrich; FBS was from Gibco; l ‐glutamine, penicillin, trypsin and streptomycin were from Euroclone; matrigel was from Corning; and gentamycin, fetuin, hEGF, bFGF and trypsin were from Life Technologies. Insulin and dexamethasone, Yoda1, gadolinium and Fura‐2 AM were from Sigma‐Aldrich; Medium 199, gentamycin, fetuin, hEGF and bFGF were from Life Technologies; and Alexa‐488‐α‐bungarotoxin was from Invitrogen.
Fetuin
Manufactured by Thermo Fisher Scientific
Sourced in United States
Fetuin is a glycoprotein found in the blood of various mammals. It functions as an inhibitor of calcium phosphate mineralization, playing a role in the regulation of biomineralization processes.
Automatically generated - may contain errors
Lab products found in correlation
Human epidermal growth factor (hegf), by Thermo Fisher Scientific (3 mentions)
Medium 199, by Thermo Fisher Scientific (2 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)
Basic fibroblast growth factor (bfgf), by Thermo Fisher Scientific (2 mentions)
Fura 2 am, by Merck Group (1 mentions)
Alexa 488 α bungarotoxin, by Thermo Fisher Scientific (1 mentions)
Matrigel, by Corning (1 mentions)
Trypsin, by Thermo Fisher Scientific (1 mentions)
Gentamycin, by Thermo Fisher Scientific (1 mentions)
Gadolinium, by Merck Group (1 mentions)
Trypsin, by Euroclone (1 mentions)
Penicillin, by Euroclone (1 mentions)
Streptomycin, by Euroclone (1 mentions)
Yoda1, by Merck Group (1 mentions)
L glutamine, by Euroclone (1 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)
Medium 199, by Merck Group (1 mentions)
Dexamethasone (dex), by Merck Group (1 mentions)
Insulin, by Merck Group (1 mentions)
Penicillin streptomycin, by Wisent (1 mentions)
3 protocols using fetuin
1
Cell Culture Reagents and Compounds
2
Immortalized Myoblasts From FSHD Patient
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Human immortalized myoblasts were derived from a healthy individual (AB1190) and from a patient with the FSHD (AB1080) (37 (link)) and were a gift of V. Mouly (Institute of Myology, Paris, France). AB1080 and AB1190 were cultured in a proliferation medium composed of four parts of high-glucose Dulbecco’s modified Eagle’s medium (DMEM) and one part of Medium 199 (Sigma-Aldrich, #M4530) supplemented with 20% fetal bovine serum (FBS) (Life Technologies, #10270), fetuin (25 μg/ml; Life Technologies, #10344026), human epidermal growth factor (hEGF) (5 ng/ml; Life Technologies, #PHG0311), basic fibroblast growth factor (0.5 ng/ml; Life Technologies, #PHG0026), insulin (5 μg/ml; Sigma-Aldrich, #91077C-1G), dexamethasone (0.2 μg/ml; Sigma-Aldrich, #D4902), and 1% penicillin-streptomycin (Gibco, #15140-122).
3
Culture Conditions for Cell Lines
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HEK293T cells and human fibroblasts were cultured in DMEM medium (Wisent Inc., Saint-Jean-Baptiste, QC, Canada) supplemented with 10% FBS (Wisent Inc.) and 1% penicillin–streptomycin (Wisent Inc.). Cells were kept at 37 °C with 5% CO2 in a humidified incubator.
The human myoblasts were cultured in a homemade medium made of DMEM medium with 20% FBS (Wisent Inc.), 16% of medium 199 (Invitrogen™ Inc., Carlsbad, CA, USA) and 1% penicillin–streptomycin (Wisent Inc.), supplemented with Fetuin 25 μg/mL (Life Technologies, Carlsbad, CA, USA), hEGF: 5 ng/mL (Life Technologies), bFGF: 0.5 ng/mL (Life Technologies), insulin: 5 μg/mL (Sigma-Aldrich Canada Inc., Oakville, ON, Canada, 91077C-1G) and Dex: 0.2 μg/mL (Sigma-Aldrich Canada Inc.).
The human myoblasts were cultured in a homemade medium made of DMEM medium with 20% FBS (Wisent Inc.), 16% of medium 199 (Invitrogen™ Inc., Carlsbad, CA, USA) and 1% penicillin–streptomycin (Wisent Inc.), supplemented with Fetuin 25 μg/mL (Life Technologies, Carlsbad, CA, USA), hEGF: 5 ng/mL (Life Technologies), bFGF: 0.5 ng/mL (Life Technologies), insulin: 5 μg/mL (Sigma-Aldrich Canada Inc., Oakville, ON, Canada, 91077C-1G) and Dex: 0.2 μg/mL (Sigma-Aldrich Canada Inc.).
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