Kasumi 1

The SKNO‐1, Kasumi‐1, and U937 cell lines were obtained from the Japanese Collection of Research Bioresources (JCRB) Cell Bank. The KG‐1a cell line was sourced from the RIKEN cell bank. SKNO‐1 was maintained in RPMI 1640 media containing 10% serum and 10 ng/ml human granulocyte‐macrophage colony‐stimulating factor (GM‐CSF; Peprotech). Kasumi‐1, U937, and KG‐1a were cultured in RPMI 1640 containing 10% serum.

SKM-1 (MDS cell line), MOLM-14 and Kasumi-1 (AML cell lines), and NIH3T3 (Mouse fibroblast-like cell line) cells were purchased from the Japanese Collection of Research Bioresources Cell Bank (Ibaraki, Osaka, Japan). U937, THP-1, and MV4-11 (AML cell lines) cells and the human marrow stromal cell line HS-5 were purchased from the American Type Culture Collection (Manassas, VA, USA). MDSL (MDS cell line) cells were kindly provided to us by Professor Kaoru Tohyama (Kawasaki Medical School, Kurashiki City, Okayama, Japan). These cell lines were grown in Roswell Park Memorial Institute 1640 (RPMI 1640) medium, which contained 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin. MDS-L cells were cultured in RPMI 1640 medium with 20% FBS. NIH3T3 and HS-5 cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) with 10% FBS.

Human acute myeloblastic leukemia cell lines GDM-1, CESS, HL-60, and QIMR-WIL were purchased from ATCC (USA), Kasumi-1, P31/FUJ were purchased from Japanese Collection of Research Bioresources Cell Bank (Japan). Manufacturers authenticated the cell lines. P31/FUJ, GDM-1, HL-60, and CESS were cultured in RPMI-1640 (Sigma, (USA)) supplemented with FBS (% 10), P/S (% 1), and 200 mM L-glu (% 1). Kasumi-1 was cultured in RPMI-1640 supplemented with FBS (% 20). QIMR-WIL was cultured in DMEM (Sigma, (USA)) supplemented with FBS (% 10), P/S (% 1), and 200 mM L-glu (% 1). All cell lines were incubated at 5% CO₂ and 37 °C in a humidified incubator.