Chromatographic separations were performed on an ACQUITY™ UPLC System (Waters Corporation, Milford, MA). A BEH C18 reversed-phase column (100 × 2.1 mm, 1.7 μm, Waters, MA, USA) and a BEH C18 guard column (5 × 2.1 mm, 1.7 μm) were used. The column was maintained at 37 °C with a flow rate of 0.4 mL/min. Mobile phase A was 0.1 % formic acid (Sinopharm, Shanghai, PRC), while mobile phase B was acetonitrile (Sinopharm, Shanghai, PRC) modified by addition of 0.1 % formic acid. Each sample was run twice: once in positive ionisation mode and once in negative ionisation mode. In positive mode, the gradient was t = 0 min, 99 % B; t = 2 min, 70 % B; t = 4 min, 25 % B; t = 7 min, 25 % B; t = 9 min, 0 % B; t = 11.5 min, 0 % B; t = 12 min, 99 % B; t = 13.5 min, 99 % B. In negative mode, the gradient was t = 0 min, 99 % B; t = 2 min, 70 % B; t = 4 min, 25 % B; t = 5 min, 25 % B; t = 7 min, 10 % B; t = 8 min, 0 % B; t = 10 min, 0 % B; t = 10.4 min, 99 % B; t = 12.2 min, 99 % B.
Beh c18 guard column
Manufactured by Waters Corporation
Sourced in United States
The BEH C18 guard column is a component used in liquid chromatography systems. Its core function is to protect the analytical column from particulate matter and contaminants, thereby extending the column's lifetime and ensuring reliable chromatographic separations.
Automatically generated - may contain errors
Lab products found in correlation
Acquity ultra performance liquid chromatograph, by Waters Corporation (2 mentions)
Tq s triple quadrupole mass spectrometer, by Waters Corporation (2 mentions)
Beh c18 column, by Waters Corporation (2 mentions)
Acquity beh c18 column, by Waters Corporation (2 mentions)
Beh c18 reversed phase column, by Waters Corporation (1 mentions)
Acquity uplc system, by Waters Corporation (1 mentions)
Acetonitrile, by Sinopharm (1 mentions)
Formic acid, by Sinopharm (1 mentions)
Acquity uplc h class, by Waters Corporation (1 mentions)
5 protocols using beh c18 guard column
1
UPLC Separation of Complex Mixtures
2
Comprehensive Metabolite Profiling by UPLC-MS/MS
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
A Waters Acquity Ultra Performance Liquid Chromatograph coupled with a Waters TQ-S triple-quadrupole mass spectrometer was used during this study. Waters MassLynx software V4.2 SCN1035 was used for the instrument control and data acquisition, and Waters TargetLynx was used to process the data. Chromatographic separation of amino acids and biogenic amines was achieved using a Waters BEH C18 column (1.7 µm, 2.1 mm × 50 mm) and Waters BEH C18 guard column (1.7 µm, 2.1 mm × 5 mm). Analysis was performed in MRM mode with positive electrospray ionization. The FIA extract was analyzed in positive mode to capture acylcarnitines, glycerophospholipids, and sphingolipids, while hexoses were monitored in negative ionization mode. Concentrations of all analytes were calculated using MetIDQ™ software Oxygen DB110-3005.
3
SCFA Quantification by LC-MS/MS
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
SCFA quantification was performed using a Waters Acquity ultra-performance liquid chromatograph coupled with a Waters TQ-S triple-quadrupole mass spectrometer. LC/MS/MS analysis was performed in negative electrospray ionization (ESI)-multiple-reaction monitoring (MRM) mode. The SCFAs were separated using a Waters BEH C18 column (1.7 µm, 2.1 mm × 50 mm) and a Waters BEH C18 guard column (1.7 µm, 2.1 mm × 5 mm). A measure of 1 mL of formic acid in 1 L water was used as mobile phase A, and 1 mL of formic acid in acetonitrile was used as mobile phase B. The flow rate of the mobile phase was set at 0.6 mL/min.
The limits of quantification (LOQ) were 10 μM for acetic acid, propionic acid, and butyric acid and 0.1 μM for isovaleric acid, valeric acid, and caproic acid.
Chromatograms of SCFAs and their internal standards (IS) of representative fecal samples are shown inSupplementary Figures S1 and S2 .
The limits of quantification (LOQ) were 10 μM for acetic acid, propionic acid, and butyric acid and 0.1 μM for isovaleric acid, valeric acid, and caproic acid.
Chromatograms of SCFAs and their internal standards (IS) of representative fecal samples are shown in
4
UPLC-PDA Analysis of Abscisic Acid
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The extract used to analyze abscisic acid (ABA) was the same as that used for the analysis of H-AOX. ABA was quantified using the UPLC Acquity H-Class (Waters) system coupled to a PDA detector (eλ detector) and Empower II software. An Acquity BEH C18 column (1.7 µm, 100 mm × 2.1 mm) (Waters) with a BEH C18 column guard (1.7 µm, 5 mm × 2.1 mm) was used. The mobile phase was composed of (A) 0.1% formic acid in MilliQ water and (B) acetonitrile with 0.1% formic acid. The gradient used was as follows: 2% B for 2 min, 2–7% B in 2 min, 7–12% B in 11 min, 12–26% B in 5 min, 26–55% B in 5 min, and 95% B for 3 min and the column was equilibrated with 2% B for 5 min. The injected volume was 10 μL with a flow of 0.2 mL/min and a column temperature of 30 °C. ABA was identified and quantified by comparing their retention time and UV-visible spectral data with a previously known injected standard (260 nm). The results were expressed in mg/kg DM.
5
Quantification of Abscisic Acid in Plant Extracts
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Abscisic acid (ABA) was quantified from the same extract used for the analysis of hydrophilic antioxidant capacity (H-AC). ABA was quantified using the UPLC Aquity H-Class (Waters) system coupled to a PDA detector (eλ detector) and Empower II software. An Acquity BEH C18 column (1.7 µm, 100 mm × 2.1 mm) (Waters) with a BEH C18 column guard (1.7 µm, 5 mm × 2.1 mm) was used. The mobile phase was composed of (A) 0.1% formic acid in MilliQ water and (B) acetonitrile with 0.1% formic acid. The gradient used was as follows: 2% of B for 2 min, 2–7% of B in 2 min, 7–12% of B in 11 min, 12–26% of B in 5 min, 26–55% of B in 5 min, and 95% B for 3 min, and the column was equilibrated with 2% B for 5 min. The injected volume was 10 μL, with a flow of 0.2 mL min−1, and a column temperature of 30 °C. ABA was identified and quantified by comparing its retention time and UV-visible spectral data to previously known injected standard (260 nm). The results were expressed in g kg−1 DM.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!