The day TAF was started was used as reference point, and HBV DNA levels were measured every 3 months during 2 years before (during ETV) and after the reference point (during TAF). Quantitative measurement of HBV DNA was done using real‐time PCR (COBAS 6800/8800 system, TaqMan HBV assay; Roche). The serum HBV DNA was measured by real‐time PCR with a detection range of 1.0–9.0 log IU/mL. The lower limit was 10 IU/mL.
Taqman hbv assay
Manufactured by Roche
The TaqMan HBV assay is a laboratory diagnostic tool used to detect and quantify the hepatitis B virus (HBV) in patient samples. The assay utilizes real-time polymerase chain reaction (PCR) technology to amplify and measure the levels of HBV genetic material, providing healthcare professionals with valuable information for the management of HBV infection.
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Lab products found in correlation
2 protocols using taqman hbv assay
1
Longitudinal HBV DNA Monitoring During TAF
2
Monitoring Hepatitis B Treatment Outcomes
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During treatment, laboratory tests were assessed every four weeks, including quantitative HBsAg, HBeAg, anti-HBs, anti-HBe, HBV DNA, hematology, clinical chemistries, and routine blood tests. The HBV DNA levels were quantified by the real-time polymerase-chain reaction assay (TaqMan HBV assay, Roche Diagnostics; lower detection limit of 50 IU/mL and higher detection limit of 3×10^8 IU/mL). The serological markers of HBV were detected with enzyme-linked immune-sorbent assay kits (ARCHITECT I 2000; Abbott Architect, USA). The biological parameters were measured using a biochemistry analyzer (7600 Series; Hitachi, Tokyo, Japan). Adverse events (AEs) are continuously monitored.
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