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Isopropanol

Manufactured by Generay
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Sourced in China

Isopropanol, also known as isopropyl alcohol or 2-propanol, is a clear, colorless, and flammable chemical compound. It is commonly used as a solvent, disinfectant, and cleaning agent in various laboratory and industrial applications.

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Lab products found in correlation

Protein marker, by Bio-Rad (1 mentions) Sodium deoxycholate, by Merck Group (1 mentions) Protein assay reagent, by Bio-Rad (1 mentions) Rosiglitazone, by Merck Group (1 mentions) Sodium dodecyl sulfate (sds), by Generay (1 mentions) Hepes, by Merck Group (1 mentions) Nahco3, by Merck Group (1 mentions) Oil red o, by Merck Group (1 mentions) Insulin, by Merck Group (1 mentions) Dexamethasone (dex), by Merck Group (1 mentions) Phenylmethylsulfonyl fluoride, by Merck Group (1 mentions) Elisa reader, by Agilent Technologies (1 mentions)

2 protocols using isopropanol

1

Adipocyte Differentiation Assay Protocol

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Insulin, rosiglitazone, dexamethasone, HEPES, NaHCO3, Oil Red O, sodium deoxycholate, and phenylmethylsulfonyl fluoride were obtained from Sigma-Aldrich (St Louis, MO, USA). SDS, Tween 20, and isopropanol were purchased from GENEray (Shanghai, China). Paraformaldehyde was obtained from TCI (Tokyo, Japan). All antibodies were purchased from Santa Cruz Biotechnology (Dallas, TX, USA). Western blotting loading buffer, Bio-Rad protein assay reagents, and protein markers were obtained from Bio-Rad (Hercules, CA, USA). Triglyceride (TG) and glucose detection kits were purchased from Asan Pharmaceutical (Seoul, Korea).
Guo L., Park S.Y., Kang H.M., Kang N.J., Hwang D.Y, & Choi Y.W. (2022). Edible Vitalmelon Fruit Extract Inhibits Adipogenesis and Ameliorates High-Fat Diet-Induced Obesity. BioMed Research International, 2022, 2369650.
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2

Quantifying Adipocyte Differentiation

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To evaluate the degree of differentiation, 150 μL isopropanol (GENEray, Shanghai, China) was used to dissolve the oil red O for each well. Next, 100 μL aliquots from each well were transferred to a new 96-well plate. The OD values then were measured at 500 nm using an ELISA reader (BioTek, Winooski, VT, USA).
Choi Y.W., Guo L., Kang J., Park Y., Je B., Hwang D., & Joo W. (2020). Fermentation of mulberry leaves with Cordyceps militaris enhanced anti-adipogenesis activity in 3T3-L1 cells through down-regulation of PPAR-γ pathway signaling. Asian Pacific Journal of Tropical Medicine, 13(12), 557-557.
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