Log In Sign up
The largest database of trusted experimental protocols

Anti yap 13584 1 ap

Manufactured by Proteintech
Visit Supplier
Sourced in China

Anti-YAP (13584-1-AP) is a primary antibody product offered by Proteintech. It is designed to detect the Yes-associated protein (YAP) in various biological samples.

Automatically generated - may contain errors

Lab products found in correlation

Anti pcna sc 25280, by Santa Cruz Biotechnology (1 mentions) Genesnap, by Syngene (1 mentions) Protease and phosphatase inhibitor, by Roche (1 mentions) Protein a g magnetic bead, by Thermo Fisher Scientific (1 mentions) Ip lysis buffer, by Thermo Fisher Scientific (1 mentions) Anti myc 2276, by Cell Signaling Technology (1 mentions) Anti taz 23306 1 ap, by Proteintech (1 mentions)

Spelling variants of this product

Anti-YAP (12 citations) YAP (13584‐1‐AP, (2 citations) Anti-TM (2 citations)

3 protocols using anti yap 13584 1 ap

1

Comprehensive Immunoblotting Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
The anti-PD-L1 (ab121545) was purchased from Abcam (Cambridge, MA, USA). The anti-p-S6 (S235/236)(4858T), anti-β-catenin (8480S), anti-Akt (4691S), anti-p-Akt (T308)(13038T), anti-p-PDK1 (Ser241)(3438T), anti-p-GSK3β (9323S), anti-p-P70S6K (T389) (9234T), anti-OCT4 (2750S), anti-pErk (4370S), anti-Erk (4695S), anti-CyclinD1 (2978S), anti-p-YAP (13008T), anti-Claudin-1 (13255T), and anti-Histone H3 (4499S) were purchased from Cell Signaling Technology (Danvers, MA, USA). The anti-WIP (sc-271113), anti-β-catenin (sc-7963), anti-MMP-9 (sc-21733), and anti-PCNA (sc-25280) were purchased from Santa Cruz (Santa Cruz, CA, USA). The anti-actin (20536-1-AP), anti-GAPDH (10494-1-AP), anti-ki67 (19972-1-AP), anti-E-cadherin (20874-1-AP), anti-N-cadherin (22018-1-AP), anti-CD44 (15675-1-AP), anti-Bax (50599-2-lg), anti-Bcl-2 (12789-1-AP), and anti-YAP (13584-1-AP) were purchased from Proteintech (Wuhan, China). The protein bands were detected by ECL according to the manufacturer’s instructions.
Yu W., Hua Y., Qiu H., Hao J., Zou K., Li Z., Hu S., Guo P., Chen M., Sui S., Xiong Y., Li F., Lu J., Guo W., Luo G, & Deng W. (2020). PD-L1 promotes tumor growth and progression by activating WIP and β-catenin signaling pathways and predicts poor prognosis in lung cancer. Cell Death & Disease, 11(7), 506.
+ Open protocol
+ Expand
2

Quantifying Protein Expression in Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Total protein was extracted, and protein concentration was measured by the BCA method. The proteins to be isolated were separated with 8% to 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis, transferred to polyvinylidene fluoride membrane, and incubated overnight at 4°C under the following primary antibodies: anti-b-TUBULIN (66240-1-Ig, Proteintech), anti-FAT1 (E95869, Sigma), anti-YAP (13584-1-AP, Proteintech), anti-P-TAZ (AF4315, Affinity), Anti-FAK (12636-1-AP, Proteintech), and anti-Src (11097-1-AP, Proteintech). Then, the membrane was incubated with anti-rabbit/mouse IgG (Abclonal, anti-rabbit: AS014, anti-mouse: AS003) secondary antibody at room temperature for 1 h, and bands developed on the membrane using Syngene G-Box and GeneSnap software (Syngene, Cambridge, UK).
Ding C., Huang H., Wu D., Chen C., Hua Y., Liu J., Li Y., Liu H, & Chen J. (2024). Pan-cancer analysis predict that FAT1 is a therapeutic target and immunotherapy biomarker for multiple cancer types including non-small cell lung cancer. Frontiers in immunology, 15.
+ Open protocol
+ Expand
3

Immunoprecipitation of YAP, TAZ, and c-Myc

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were lysed in IP lysis buffer (Thermo Fisher Scientific) containing protease and phosphatase inhibitors (Roche, Basel, Switzerland) and sonicated. Cell lysates were incubated with anti-YAP (13584-1-AP, Proteintech), anti-TAZ (23306-1-AP, Proteintech), or anti-Myc (2276S, Cell Signaling Technology) antibodies at 4°C overnight. The immunocomplexes were added to washed Protein A/G magnetic beads (88802, Thermo Fisher Scientific) and incubated at room temperature for 1 hour. Last, the beads were washed five times with IP lysis buffer and subjected to Western blot analysis.
Wang Y.Q., Wu D.H., Wei D., Shen J.Y., Huang Z.W., Liang X.Y., Cho W.C., Ma J., Lv J, & Chen Y.P. (2023). TEAD4 is a master regulator of high-risk nasopharyngeal carcinoma. Science Advances, 9(1), eadd0960.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!