Methyl esters of waste oil samples were prepared by the IUPAC method (2.301, IUPAC 1992) . GCMS (Agilent 5975 GC-MSD) instrument was used for identifying major and minor fatty acids in oil samples. The capillary column (30 m dimension) was used with helium as a carrier gas. A flow rate of carrier gas was set at 1.5 mL/min. About 2 µL of the sample was injected using split mode.
5975 gc msd
Manufactured by Agilent Technologies
The Agilent 5975 GC-MSD is a gas chromatograph-mass spectrometer system designed for qualitative and quantitative analysis of complex samples. It combines a high-performance gas chromatograph with a robust mass selective detector, enabling the separation, identification, and quantification of a wide range of organic compounds.
Automatically generated - may contain errors
Lab products found in correlation
6890n gc system, by Agilent Technologies (1 mentions)
Sodium persulphate, by Merck Group (1 mentions)
Powerwave xs, by Agilent Technologies (1 mentions)
Methanol chromasolv, by Merck Group (1 mentions)
Uv spectrophotometer, by Shimadzu (1 mentions)
Gallic acid, by Merck Group (1 mentions)
1 1 diphenyl 2 picrylhydrazyl dpph, by Merck Group (1 mentions)
5 protocols using 5975 gc msd
1
Fatty Acid Profiling of Waste Oils
2
GC-FID Analysis of Volatile Compounds
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
For gas
chromatography–flame ionization detection (GC-FID) analyses,
the FID was used at 300 °C (Agilent 6890N GC system). Simultaneous
automatic injection was carried out using the same conditions in two
identical columns in the gas chromatography–mass spectrometry
(GC/MS) system (Agilent 5975 GC-MSD). Relative percentages (%) of
the volatiles were calculated using the FID chromatograms. This process
was performed by GC/MS Library, MassFinder 3 Library, and in-house
“Baser Library of Essential Oil Constituents” by analyzing
either authentic samples or the relative retention index (RRI) of n-alkanes.41 (link),42 (link)
chromatography–flame ionization detection (GC-FID) analyses,
the FID was used at 300 °C (Agilent 6890N GC system). Simultaneous
automatic injection was carried out using the same conditions in two
identical columns in the gas chromatography–mass spectrometry
(GC/MS) system (Agilent 5975 GC-MSD). Relative percentages (%) of
the volatiles were calculated using the FID chromatograms. This process
was performed by GC/MS Library, MassFinder 3 Library, and in-house
“Baser Library of Essential Oil Constituents” by analyzing
either authentic samples or the relative retention index (RRI) of n-alkanes.41 (link),42 (link)
3
GC-MS Analysis of Organic Compounds
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
GC-MS chromatograms were recorded using Agilent Technologies 7890A and Agilent 5975 GC MSD equipped. The GC was equipped with HP-5MS column. Helium was used as carrier gas at a flow rate of 1 mL/min. Injector temperature was 250 °C. The oven temperature was programmed from 50 °C (5 min hold) to 250 °C at 10 °C/min and finally held isothermally for 15 min. For GC-MS detection, an electron ionization system, with ionization energy of 70 eV was used. A scan rate of 0.5 s (cycle time: 0.2 s) was applied, covering a mass range from 40-400 amu.
4
GC-MS Analysis of Organic Compounds
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
GC-MS chromatograms were recorded using Agilent Technologies 7890A and Agilent 5975 GC MSD equipped. The GC was equipped with HP-5MS column (30 m × 0.25 mm × 0.25 μm film thickness). Helium was used as carrier gas at a flow rate of 1 mL/min. Injector temperature was 250 °C. The oven temperature was programmed from 50 °C (5 min hold) to 250 °C at 10 °C/min and finally held isothermally for 15 min. For GC-MS detection, an electron ionization system, with ionization energy of 70 eV was used. A scan rate of 0.5 s (cycle time: 0.2 s) was applied, covering mass range was from 40-400 amu.
5
Hydrodistillation and Antioxidant Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Clevenger type apparatus for hydrodistillation (Ildam); UV-spectrophotometer (Shimadzu), microplate reader (BioTek Power Wave XS) and vortex (Heidolph reaxtop) for antioxidant assays; Gas Chromatography system (Agilent 6890 N GC) and Gas Chromatography-Mass Spectrometry (Agilent 5975 GC-MSD) were used for essential oil analyzes. Chemicals and standards: 1,1-diphenyl-2-picrylhydrazyl (DPPH • ) (Aldrich), 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) diammonium salt (ABTS) ≥ 98% (HPLC) (Sigma), Ethanol absolute (Riedel), Gallic acid (Sigma), Methanol Chromasolv (HPLC grade) (Sigma Aldrich), Sodium persulphate (Sigma), Trolox % 97 (Aldrich).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!