Donkey anti rabbit igg hrp

Manufactured by Southern Biotech

Donkey anti-rabbit IgG HRP is a secondary antibody conjugated with horseradish peroxidase (HRP). It is designed to detect and bind to rabbit immunoglobulin G (IgG) in various immunoassay applications.

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Lab products found in correlation

Anti goat irdye 800cw, by LI COR (1 mentions) Irdye 800cw, by LI COR (1 mentions) Ab8224, by Abcam (1 mentions) Ab2175, by Abcam (1 mentions) Goat anti mouse igg hrp, by Thermo Fisher Scientific (1 mentions) F1804, by Abcam (1 mentions)

2 protocols using donkey anti rabbit igg hrp

Western Blot Antibody Validation

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Primary antibodies included: anti-TREX1 (1:1,000 dilution) (Abcam, ab185228), anti-Î²-actin (1:2,000 dilution) (Abcam, ab8224), anti-RPA32 (1:1,000 dilution) (Abcam, ab2175), anti-flag (1:2,000 dilution) (Abcam, f1804) and anti-HSP60 (1:1,000 dilution) (Santa Cruz Biotechnology, sc-1052).
Secondary antibodies (1:10,000â€"15,000 dilution) included: goat anti-mouse IgG HRP (Thermo Fisher Scientific, 31432), donkey anti-rabbit IgG HRP (SouthernBiotech, 6441-05), anti-rabbit secondary antibody IRDye 800 CW (LI-COR Biosciences, 926-32213) and anti-goat IRDye 800 CW (LI-COR Biosciences, 926-32214).

Karasu M.E., Toufektchan E., Chen Y., Albertelli A., Cullot G., Maciejowski J, & Corn J.E. (2024). Removal of TREX1 activity enhances CRISPR-Cas9-mediated homologous recombination. Nature biotechnology.

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Immunoblotting for Sj-p80 and E. coli HCP

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For the Sj-p80 immunoblotting, a cross-reactive anti-Sm-p80/Sj-p80 monoclonal antibody (Mab) (SMab4) was selected for use. Proteins were resolved by SDS-PAGE, transferred to PVDF membrane, blocked, and then probed with SMab4 at a concentration of 0.013 µg/ml for 1 h. Bound Mab was detected using goat-anti-mouse IgG (H+L) conjugated to horseradish peroxidase (HRP) (Southern Biotech, Birmingham, AL) at a dilution of 1:2000 and exposed with Ultra-TMB (Promega, Madison, WI). For the anti-E. coli HCP Western, blots were probed for 1 h with Rabbit-anti-Ec-HCP polyclonal antisera (Rockland, Limerick, PA) at a dilution of 1:1000 and bound antibody was detected using Donkey-anti-rabbit IgG-HRP (Southern Biotech) at a dilution of 1:2000.

Molehin A.J., Gray S.A., Turner C., Davis J., Zhang W., Khatoon S., Rattan M., Kernen R., Peterson C., Sennoune S.R., Carter D, & Siddiqui A.A. (2021). Process Development of Sj-p80: A Low-Cost Transmission-Blocking Veterinary Vaccine for Asiatic Schistosomiasis. Frontiers in Immunology, 11, 578715.

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