All reagents, including 2-aminobenzenethiol, p-hydroxybenzaldehyde, toluene, acetic acid, 1, 3-dimercaptopropane and hexamethylenetetramine, were analytical grade and purchased from commercial supplies without further purification. NMR spectra were performed on Bruker 500 MHz instruments (AVANCE IIITM 500). MS spectrum was obtained on LTQ-Orbitrap-ETD high resolution mass spectrometer (Thermo Scientific, Orbitrap Elite). Live cell imaging was carried out on Olympus fluorescent microscope (Fluo View FV1000). UV-vis absorption and fluorescence spectral studies were recorded on an Evolution 220 UV-Visible (Thermo) and FluroMax-4 Spectrophotometer (HORIBA Scientific), respectively.
Evolution 220 uv visible
Manufactured by Thermo Fisher Scientific
The Evolution 220 UV-Visible is a spectrophotometer that measures the absorption of ultraviolet and visible light by samples. It is designed to provide accurate and reliable results for a variety of applications.
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Lab products found in correlation
2 protocols using evolution 220 uv visible
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Fluorescent Probe Synthesis and Imaging
2
Curcumin Extraction from Turmeric Rhizomes
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Rhizomes of turmeric were collected from field before onset of dormancy (i.e., November) for extraction of curcumin. The rhizomes were cleaned thoroughly with water, cut into small pieces and air dried. The air dried rhizomes were powdered in mortar with a pestle, 0.1 g of powdered rhizome was taken in a flat bottom flask and 75 ml of acetone was added and refluxed for 4 h. The refluxed residue was cooled, filtered and washed with 100 ml of acetone. The absorbance of the diluted sample and that of the standard curcumin (95% HPLC Purified, Charak) solution were measured at 420 nm by spectrophotometer (Thermo Scientific, Evolution 220 UV Visible) and curcumin percentage of the sample was estimated according to the ASTA method (American Spice Trade Association, 1997 ).
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