Sigma bilirubin assay kit
The Sigma Bilirubin Assay kit is a laboratory equipment product designed to measure bilirubin levels in biological samples. It provides a quantitative determination of total bilirubin, direct bilirubin, and indirect bilirubin concentrations.
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2 protocols using sigma bilirubin assay kit
Serum Biochemistry Profiling in Mice
Serum Biochemistry Analysis in Mice
For non-terminal procedures about 200 µL was collected otherwise about 600 µL was collected.
Blood from the capillary was transferred into BD microtainer tubes and then centrifuged according to manufacturer's protocol to separate the serum. Serum was then transferred into 1.5 mL microcentrifuge tubes and snap-frozen in liquid nitrogen before storing at -80 °C. Serum cholesterol, triglycerides, ALT, and AST activity were measured using colorimetric assay kits provided by Infinity (Thermo Scientific). Serum bilirubin, both direct and indirect, was measured using the Sigma Bilirubin Assay kit (MAK126 Sigma) according to the manufacturer's protocol.
Snap frozen serum (100 µL) collected from control and acSRSF1 HKO mice were submitted to the Mouse Metabolic Phenotyping Core at the University of Cincinnati for serum analysis. A serum chemistry panel was obtained for each sample with measured concentrations of serum triglycerides, cholesterol, phospholipids, non-esterified fatty acids, glucose, ketones, ALT, and AST.
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