A1r hd inverted confocal microscope

A Nikon A1R HD inverted confocal microscope at the University of Notre Dame Integrated Imaging Facility was used to capture serial optical sections of retinal slices using 40x and 60x oil-immersion objectives. Z-stacks of ~10 µm from the central dorsal retinal tissue were captured using NIS-Elements (RRID:SCR_014329). The resulting images were analyzed as either single optical sections or maximum z-stack projections (where appropriate) using ImageJ/FIJI^{73 (link)}. For the colocalization experiments, single optical sections were used. We increased the saturation of the Gfap and 4c12 channels to increase the cell body staining to determine colocalization with EdU.

A Nikon A1R HD inverted confocal microscope at the University of Notre Dame Integrated Imaging Facility was used to capture serial optical sections of retinal slices using 40x and 60x oil-immersion objectives. Z-stacks of approximately 10μM from the central dorsal retinal tissue were captured using NIS-Elements (RRID:SCR_014329). The resulting images were analyzed as either single optical sections or maximum z-stack projections (where appropriate) using ImageJ/FIJI ^{68 (link)}. For the colocalization experiments, single optical sections were used. We increased the saturation of the Gfap and 4c12 channels to increase the cell body staining to determine colocalization with EdU.