F 12 nutrient mixture ham ham s f 12

Salmon calcitonin was purchased from Bachem AG (Bubendorf, Switzerland). Penetratin (RQIKIWFQNRRMKWKK) and FITC labeled Penetratin (FITC-Penetratin) were purchased from Peptron Co., Ltd. (Daejeon, Korea). Alexa Fluor 647 NHS Ester, SnakeSkin^TM dialysis tubing (3.5 K MWCO), Fetal bovine serum (FBS), 0.25% trypsin-EDTA and penicillin/streptomycin were purchased from Thermo Fisher Scientific (Waltham, MA, USA). The sCT ELISA kit was purchased from Phoenix Pharmaceuticals (Burlingame, CA, USA). F-12 Nutrient mixture Ham (Ham’s F-12) was purchased from Welgene (Gyeongsan-si, Korea). The CellTiter 96^® Aqueous One Solution Cell Proliferation Assay kit (MTS) was purchased from Promega (Madison, WI, USA). All other chemicals and solvents were reagent grade.

Fetal bovine serum (FBS) and antibiotic-antimycotic (100×) were procured from
Gibco (Waltham, MA), and phosphate-buffered saline (PBS) and F-12 Nutrient
Mixture Ham (Ham’s F-12) were purchased from WELGENE Inc (Daegu, Korea). Annexin
V-fluorescein isothiocyanate (FITC) Apoptosis Detection Kit was obtained from
Sigma-Aldrich (St. Louis, MO). Whole-cell lysis buffer was procured from iNtRON
Biotechnology Inc (Seoul, Korea). Antibodies against B-cell lymphoma Bak, Bcl-2,
Bcl-xL, caspase-3, and caspase-9 were supplied by Cell Signaling Technology
(Beverly, MA), and those against GLI2 and β-actin were obtained from Santa Cruz
(Dallas, TX). GLI1, PTCH-1, and SMO antibodies used for immunocytochemistry were
purchased from Abcam (Cambridge, UK).