Pe mouse anti human cd18

Manufactured by BD

The PE mouse anti-human CD18 is a laboratory reagent used for the identification and analysis of CD18-positive cells in human samples. It is a fluorochrome-conjugated monoclonal antibody that binds specifically to the CD18 antigen expressed on the surface of various cell types.

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2 protocols using pe mouse anti human cd18

Phenotypic Characterization of OASCs

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Related cell markers of OASCs were analyzed by flow cytometry. The dissociated cells were incubated with fluorescein isothiocyanate (FITC) mouse anti-human CD29, phycoerythrin (PE) mouse anti-human CD34, PE mouse anti-human CD18, FITC mouse anti-human CD49e, PE mouse anti-human CD166, allophycocyanin (APC) mouse anti-human CD133, PE mouse anti-human CD45, and APC mouse anti-human CD105 (BD Biosciences) respectively at 4 °C for 30 min, washed, and resuspended with PBS. The cells then underwent flow cytometry using the BD FACS Calibur. Analysis was performed using the Flow-Jo program (Treestar, USA).

Sun P., Shen L., Zhang C., Du L, & Wu X. (2017). Promoting the expansion and function of human corneal endothelial cells with an orbital adipose-derived stem cell-conditioned medium. Stem Cell Research & Therapy, 8, 287.

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Quantifying CSC and CXCR1 Populations

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CSC populations (CD24⁻/CD44⁺ and ALDH⁺) were measured by flow-cytometry of single-cell suspensions obtained from tumor biopsies (Additional file 1: Supplemental Materials) by using anti-human CD44-APC (clone G44-26) and CD24-FITC (clone ML5, RUO) antibodies (BD Biosciences, Franklin Lakes, NJ), and ALDEFLUOR assay (StemCell Technologies, Inc., Vancouver, BC, Canada), respectively, as previously published [13 (link)].
Additional analyses were directed to determine CXCR1 (PerCP/Cy5.5 mouse anti-human CD181 (CXCR1; BioLegend, San Diego, CA) and cell lineages using the following antibodies: APC mouse anti-human CD44 (BD Bioscience, San Jose, CA; catalog #559942), PE-Cy7 mouse anti-human CD24 (#561646), PE mouse anti-human CD2 (#555327), PE mouse anti-human CD31 (#555446), PE mouse anti-human CD3 (#555333), PE mouse anti-human CD18 (#555924), PE mouse anti-human CD16 (#555407), PE mouse anti-human CD19 (#555413), and PE mouse anti-human CD45 (#555483).

Goldstein L.J., Perez R.P., Yardley D., Han L.K., Reuben J.M., Gao H., McCanna S., Butler B., Ruffini P.A., Liu Y., Rosato R.R, & Chang J.C. (2020). A window-of-opportunity trial of the CXCR1/2 inhibitor reparixin in operable HER-2-negative breast cancer. Breast Cancer Research : BCR, 22, 4.

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