The mouse NSCLC cell line LLC was obtained from our laboratory. Hygromycin B (1 μg/ml) was added to shFGFR1 and the control shRNA. Healthy male C57BL/6 mice aged 6-8 w were inoculated with FGFR1 knockdown or wild-type LLC cells. The cells (50*10^4) were digested, washed, and resuspended in 100 μL PBS before being injected into the left armpit of each mouse. Beginning on day 6, the size of each tumor was measured with calipers every three days. The subcutaneous tumor volume was calculated as (L2×W)/2, where V is the tumor volume, L is the longest diameter, and W is the shortest diameter. InVivoMab anti-mouse PD-1 (Bio X Cell, USA) or rat IgG2α isotype (Bio X Cell, USA) was injected intraperitoneally at a dose of 100 μg per mouse on day 6, 8, 10 and 12. The mice were sacrificed on day 15. The tumors were removed and then imaged and analyzed. The tissues were digested with collagenase A (0.33 U/ml), dispase (0.85 U/ml) and DNase I (144 U/ml) with rapid shaking at 37 °C. Large pieces of debris were removed by successive filtration through 70-µm and 40-µm strainers.
Rat igg2α isotype
Manufactured by BioXCell
Sourced in United States
Rat IgG2α isotype is a type of immunoglobulin G antibody found in rats. It is commonly used in research applications as a control or reference standard.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using rat igg2α isotype
1
Investigating FGFR1 in Murine NSCLC
2
Investigating FGFR1 in Murine NSCLC
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The mouse NSCLC cell line LLC was obtained from our laboratory. Hygromycin B (1 μg/ml) was added to shFGFR1 and the control shRNA. Healthy male C57BL/6 mice aged 6-8 w were inoculated with FGFR1 knockdown or wild-type LLC cells. The cells (50*10^4) were digested, washed, and resuspended in 100 μL PBS before being injected into the left armpit of each mouse. Beginning on day 6, the size of each tumor was measured with calipers every three days. The subcutaneous tumor volume was calculated as (L2×W)/2, where V is the tumor volume, L is the longest diameter, and W is the shortest diameter. InVivoMab anti-mouse PD-1 (Bio X Cell, USA) or rat IgG2α isotype (Bio X Cell, USA) was injected intraperitoneally at a dose of 100 μg per mouse on day 6, 8, 10 and 12. The mice were sacrificed on day 15. The tumors were removed and then imaged and analyzed. The tissues were digested with collagenase A (0.33 U/ml), dispase (0.85 U/ml) and DNase I (144 U/ml) with rapid shaking at 37 °C. Large pieces of debris were removed by successive filtration through 70-µm and 40-µm strainers.
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