2 2 0 azobisisobutyronitrile aibn

MPC was purchased from NOF Co., Tokyo, Japan, which was synthesized using a reported procedure. 25, 26 BMA was obtained from FUJI FILM Wako Pure Chemical Co., Ltd (Osaka, Japan). VPBA and 2,2 0 -azobisisobutyronitrile (AIBN) were obtained from Kanto Chemical Co., Inc. (Tokyo, Japan). PVA (NH-18S, polymerization degree of 2100) was obtained from Nippon Synthetic Chemical Industry Co., Ltd (Tokyo, Japan). A waterinsoluble poly(MPC-co-BMA) (Lipidure-PMB) was purchased from NOF Co. 26 Human recombinant bone morphogenetic protein-2 (BMP-2) was purchased from R&D Systems, Inc. (Minneapolis, MN, USA). Dulbecco's modified Eagle's medium (DMEM), fetal bovine serum (FBS), and phosphate-buffered saline (PBS) were purchased from Thermo Fisher Scientific (Waltham, MA, USA). Dulbecco's modified Eagle's medium (DMEM) containing 10% FBS and 1.0% penicillin was prepared. Trypsin (0.05% solution) containing 1 mmol L À1 N,N,N 0 ,N 0 -ethylenediamine tetraacetic acid (EDTA) was purchased from Invitrogen (Tokyo, Japan). C3H10T1/2 cells were purchased from RIKEN BRC through the National Bio-Resource Project of MEXT, Japan. Other reagents and solvents were commercially available extra-pure grade reagents and were used without further purification.

A PEEK sheet (APTIV1000 sheet, 100 mm thick) was obtained from Victrex PLC (Lancashire, UK). MPC and Lipidure s -PMB (water-soluble random PMB) were purchased from NOF Co., Ltd (Tokyo, Japan), which was synthesized using a previously reported procedure. 27 Additionally, 2,2 0 -azobisisobutyronitrile (AIBN) was purchased from Kanto Chemical Co., Inc. (Tokyo, Japan). Ethylene bis(2-bromoisobutyrate) (EBBiB), tris(2pyridylmethyl) amine (TPMA), and 4-cyano-4-(phenylcarbonothioylthio)pentanoic acid (CPA) were purchased from Sigma-Aldrich (St Louis, MO, USA). L-Ascorbic acid, copper(II) bromide (CuBr 2 ), and albumin from bovine serum (BSA, 019-21272) were obtained from Wako Pure Chemical Industries (Osaka, Japan). All reagents were of extra-pure grade and were used without further purification.