Cell death were assessed using a NovoStar multiwell plate reader (BMG Lab Technologies, Offenburg, Germany), as previously described (Nieminen et al., 1992 (link)). Briefly, after attachment to 24-well plates for 3 h, hepatocytes were washed once and HDM containing 30 μM PI (Invitrogen, Eugene, OR) was added. Hepatocytes were then incubated with 10 mM APAP. In some experiments, hepatocytes were treated with 4 μM minocycline and/or 20 mM NAC at 1 h before APAP or at 2 or 4 h after APAP. PI fluorescence from each well was measured at excitation and emission wavelengths of 544 nm and 620 (40-nm bandpass), respectively. For each well, fluorescence was first measured at 20 min after addition of PI (Initial) and then at various times after treatment of APAP (X). Experiments were terminated by permeabilizing plasma membranes with 375 μM digitonin. After another 20 min, a final fluorescence measurement (Final) was collected. The percentage of nonviable cells (D) was calculated as D = 100(X -Initial)/(Final - Initial). Cell killing assessed by PI fluorimetry correlates closely with trypan blue exclusion and enzyme release as indicators of oncotic necrosis (Nieminen et al., 1992 (link); Kim et al., 2003 (link)).
Novostar multiwell plate reader
Manufactured by BMG Labtech
Sourced in Germany
The NovoStar is a multiwell plate reader designed for high-throughput screening and quantitative analysis. It offers a range of detection modes, including absorbance, fluorescence, and luminescence, enabling a variety of applications in life science research and drug discovery. The NovoStar provides accurate and reliable data, ensuring consistent results across multiple experiments.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using novostar multiwell plate reader
1
Quantifying Hepatocyte Cell Death
2
Measuring Corticosterone in Rat Stress Response
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
To measure corticosterone (CORT), one of the primary stress hormone in rats, blood samples were collected by tail nick during the 20th minute of restraint or control procedures. This sampling occurred between 9–11 AM, when levels are near their basal diurnal state. Blood (approximately 300 µL) was placed in a tube containing heparin, and immediately placed on ice, then centrifuged at 3200 rpm for 5 min at 4°C. Clear plasma was pipetted out, put into tubes and frozen at −20°C until analysis. Samples were analyzed by enzyme immunoassay for corticosterone (Corticosterone EIA kit, Cayman Chemicals), following the manufacturer's suggested protocol. Absorbance was measured and quantified against known concentrations of corticosterone (405 nm; Novostar multiwell plate reader, BMG LabTechnologies, Ortenberg, Germany). All samples were run in duplicate. Corticosterone concentration in samples were interpolated from an exponential fit to percent bound ÷ maximal bound (%B/Bmax) curve from known concentrations and expressed as pg/mL. For calculation, absorbance measures of blank controls and non-specific binding controls were subtracted out from all values.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!