Mda mb 231

Breast cancer (MDA-MB231, MCF-7) and colon cancer (LS180, SW480) cell lines were obtained from the ATCC (Manassas, VA). The pancreatic cancer cell lines (PATU8988T, Panc1) were kindly provided by Dr. A. Kimmelman (Harvard Medical School, Boston, MA). The T2 cell line was provided by Dr. J. Molldrem (University of Texas M. D. Anderson Cancer Center, Houston, TX). MDA-MB231 and SW480 cell lines were cultured in Leibovitz’s L-15 Medium (ATCC); MCF-7 and LS180 cell lines were cultured in EMEM (Gibco-Life Technologies, Rockville, MD); and Panc1, PATU8902, and T2 cell lines were cultured in DMEM (Gibco-Life Technologies) media. All media were supplemented with 10% fetal calf serum (FCS; BioWhittaker, Walkersville, MD), 100 IU/ml penicillin, and 100 μg/ml streptomycin (Gibco-Life Technologies).

The MM cell lines, MM1S, OPM2, OPM1, H929, OCIMY5, RPMI, U266, KMS1, HSB2, McCAR and ANBL6, and a breast cancer cell line MDA-MB-231 were obtained from ATCC (Manassas, VA). The T2 cell line, a human B and T cell hybrid expressing HLA-A2 molecules, was provided by Dr. J. Molldrem (University of Texas M. D. Anderson Cancer Center, Houston, TX). The cell lines were cultured in DMEM (for MM and T2 cells; Gibco-Life Technologies, Rockville, MD) or Leibovitz’s L-15 (for MDA-MB231; ATCC, Manassas, VA) media supplemented with 10% fetal calf serum (FCS; BioWhittaker, Walkersville, MD), 100 IU/ml penicillin and 100 µg/ml streptomycin (Gibco-Life Technologies).

J774A.1 (murine macrophage), Hep G2 (human hepatocellular liver carcinoma) and MDA-MB-231 (human breast cancer) cells were purchased from ATCC (Manassas, VA, USA). The Hep G2 cells were cultured in Eagle's Minimum Essential Medium, 10% fetal bovine serum (Gibco, Grand Island, NY USA), 45 IU/ml penicillin and 45 IU/ml streptomycin (Gibco). The J774A.1 and MDA-MB-231 cells were maintained in a culture medium of DMEM (ATCC), 10% FBS, 45 IU/ml penicillin and 45 IU/ml streptomycin (Gibco). The cells were grown at 37 °C in 5% CO₂ humidified incubator.

MCF-7 (ER+/PR+/HER2−, BRCA1 proficient), MDA-MB-231 (ER−/PR−/HER2−, BRCA1 proficient), MDA-MB-468 (ER−/PR−/HER2−, BRCA1 proficient) and MDA-MB-436 (ER−/PR−/HER2−, BRCA1 deficient) were purchased from ATCC and were grown in RPMI (MCF-7) or DMEM (MDA-MB-231, MDA-MB-468 and MDA-MB-436) medium with the addition of 10% foetal bovine serum and 1% penicillin/streptomycin. Cells in culture were routinely checked for mycoplasma contamination by PCR (Sigma, catalog no. MP0035).The cells characterisation were performed by ATCC and passaged in the laboratory for fewer than 6 months.

MDA-MB-231 (HTB-26), MDA-MB-468 (HTB-132), MCF10A (CRL-10317), and MCF-7 (HTB-22) were purchased from ATCC in 2010 (MCF-7), 2012 (MDA-MB-468), and 2014 (MDA-MB-231 and MCF10A). Primary human fibroblast cells were kindly provided by Kathrin Scheckenbach, Düsseldorf, Germany in 2014. TMD231 cells were obtained from Dr. Harikrishna Nakshatri in 2009 and are a derivative of the MDA-MB-231 cell line (19 (link)). TMD231 cells were transduced with E2-Crimson lentiviral vector, p2CL7CR2wo, (TMD231-CR) for in vivo imaging as described (20 (link)). Upon receipt, cell stocks were cryopreserved at low passage. Authentication of molecular profiles was verified by short tandem repeat (STR) analysis (IDEXX BioResearch), and cells tested negative for mycoplasma. TMD231 cells were cultured in MEM-α (Gibco) supplemented with 10% FBS (Atlanta Biologicals) and 1% HEPES (Invitrogen). MDA-MB-231, MDA-MB-468, and primary human fibroblast cells were cultured in DMEM (Gibco) supplemented with 10% FBS (Atlanta Biologicals). MCF10A cells were cultured in Medium 171 (Gibco) supplemented with 1% MEGS (Invitrogen) and 0.1% cholera toxin (Sigma). All cells were cultured at 37°C with 5% CO₂.