Human renal cell carcinoma cell lines: Caki-1(metastatic clear cell renal cell carcinoma), Caki-2 (primary clear cell renal cell carcinoma) and ACHN (metastatic papillary renal cell), were purchased from the American Type Culture Collection (ATCC Manassas, VA). Caki-1 and Caki-2 were maintained with McCoy's 5A medium (ATCC Manassas, VA) containing 10% Fetal Bovine Serum (FBS) (Gibco Grand Island, NY) and ACHN was maintained in RPMI 1640 medium (HyClone Logan, UT) containing 10% FBS and L-glutamine (Gibco Grand Island, NY). Human umbilical vascular endothelial cells (HUVEC) were also obtained from ATCC and maintained with EBM-2 PLUS medium (Lonza Basel, CH). Co-culture was performed using 6 well 3.0um pore sized PET membrane cell inserts (Corning New York, NY) and cells were co-cultured in a 50/50 mix of each individual Medium. All cells were cultured at 37°C in a humid atmosphere with 5% CO2. Mycoplasma contamination was tested every week.
Caki 1
Manufactured by American Type Culture Collection
Sourced in United States
The Caki-1 is a laboratory equipment product offered by the American Type Culture Collection. It is a cell line derived from a human carcinoma. The Caki-1 cell line is used for research purposes, but a detailed description of its core function is not available while maintaining an unbiased and factual approach.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (96 mentions)
Caki 2, by American Type Culture Collection (78 mentions)
Osrc 2, by American Type Culture Collection (54 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (45 mentions)
Caki 1, by Thermo Fisher Scientific (39 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (36 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (34 mentions)
Rpmi 1640, by Thermo Fisher Scientific (23 mentions)
Penicillin, by Thermo Fisher Scientific (20 mentions)
Hek293t, by American Type Culture Collection (18 mentions)
Streptomycin, by Thermo Fisher Scientific (18 mentions)
Hek293, by American Type Culture Collection (14 mentions)
Lipofectamine 2000, by Thermo Fisher Scientific (14 mentions)
Sw839, by American Type Culture Collection (13 mentions)
Mccoy s 5a medium, by Thermo Fisher Scientific (11 mentions)
Dulbecco s modified eagle s medium, by Thermo Fisher Scientific (10 mentions)
Lipofectamine 3000, by Thermo Fisher Scientific (8 mentions)
Human umbilical vein endothelial cells (huvec), by American Type Culture Collection (7 mentions)
Lipofectamine 2000 reagent, by Thermo Fisher Scientific (7 mentions)
Fetal bovine serum (fbs), by Merck Group (7 mentions)
345 protocols using caki 1
1
Renal Cell Carcinoma Cell Lines Co-culture
2
Cell Lines for Renal Cancer Research
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The normal RPTEC (ATCC number: CRL-4031) and renal cancer ACHN (ATCC number: CRL-1611) and Caki1 (ATCC number HTB-46) cell lines were purchased from the ATCC (Manassas, VA). These human-derived cell lines were authenticated by DNA short-tandem repeat analysis by ATCC. Cell line experiments were performed within 5–6 months of their procurement/resuscitation. ACHN cells were cultured in MEM media, Caki1 cells in and McCoy 5A medium, and RPTEC cells in DMEM:F12 Medium (ATCC® 30-2006™). All media were supplemented with 10% FBS and 1X antibiotics (penicillin and streptomycin). Cell lines were maintained at 37 °C and humidified atmosphere of 5% CO2.
3
Culturing clear cell renal carcinoma cell lines
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The human ccRCC cell lines 786-O (ATCC® CRL-1932™) and Caki-1 (ATCC® HTB-46™) (American Type Culture Collection, Manassas, VA, USA) were cultured under standard conditions (37°C, 5% CO2). 786-O, a VHL mutant cell line with altered HIF and VEGF pathways derived from a primary epithelial clear cell adenocarcinoma [37 (link)], was maintained in RPMI 1640 (ATCC). Caki-1, a VHL wild type cell line that is derived from a metastatic site on the skin and is characterized by high VEGF production [37 (link)], was grown in McCoy’s 5A media (ATCC). All media were supplemented with 10% fetal bovine serum, penicillin/streptomycin, and L-glutamine.
4
Culturing Kidney Cell Lines for Research
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RCC cell lines including ACHN, A-498, 786-O, and Caki-1 and the human normal kidney tubule epithelial cell line HK-2 were purchased from the American Type Culture Collection (ATCC; Manassas, VA, USA). HK-2 cells were cultured in keratinocyte serum-free medium (Gibco, Rockville, MD, USA) supplemented with 0.05 mg/ml bovine pituitary extract and 0.5 ng/ml human recombinant epidermal growth factor. ACHN and A-498 cells were cultured in Eagle’s minimum essential medium (ATCC); 786-O cells were grown in Roswell Park Memorial Institute (RPMI)-1640 medium (Gibco); and Caki-1 cells were cultured in McCoy’s 5a modified medium (ATCC). In addition, 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin mix (Sigma-Aldrich, St. Louis, MO, USA) were added to the media. Cells were grown in a humidified atmosphere of 95% air and 5% CO2 at 37°C.
5
Cell Culture of Renal Cell Carcinoma
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The human ccRCC cell lines 786-O (ATCC® CRL-1932™) and Caki-1 (ATCC® HTB-46™) (American Type Culture Collection, Manassas, VA, USA) were cultured under standard conditions (37 °C, 5% CO2). A VHL mutant primary epithelial clear cell adenocarcinoma with altered HIF and VEGF pathways [35 (link)], 786-O, was grown in RPMI 1640 (ATCC). Caki-1, a VHL wild type model line of metastatic ccRCC with high VEGF production [35 (link)], was maintained in McCoy’s 5A media (ATCC). All media were supplemented with 10% fetal bovine serum, penicillin/streptomycin, and L-glutamine.
6
In vitro RCC Cell Line Characterization
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Human RCC cell lines, characterized as an adenocarcinoma (ACHN) and clear cell carcinomas (Caki-1 and 769-P), were originally purchased from ATCC [25 –27 (link)]. All cells were cultured in RPMI with 10% FBS, 1% glutamine, and 1% Pen/Strep. For in vitro experiments, cells were seeded on the appropriated plates overnight and treated with HCQ (75 or 100 μM, Acro Chemicals), RAD001 (10 μM, LC Laboratories), bafilomycin A1 (50 nM, Sigma), or spautin-1 (10 μM, Sigma) for 48 hours. Bortezomib (Velcade, 1 μ g/ml) was obtained from the Fox Chase Cancer Center pharmacy and treated cells for 16 hours.
7
Culturing Clear Cell Renal Carcinoma Cell Lines
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The human ccRCC lines 786-O (ATCC® CRL-1932™) and Caki-1 (ATCC® HTB-46™) (American Type Culture Collection, Manassas, VA, USA) were cultured under standard conditions (37 °C, 5% CO2). 786-O, a VHL mutant RCC cell line with altered HIF and VEGF pathways, was derived from a primary epithelial clear cell adenocarcinoma and maintained in RPMI 1640 (ATCC). Caki-1, derived from a metastatic site on the skin, is a VHL wild type RCC cell line characterized by high VEGF production, and was grown in McCoy’s 5A media (ATCC). All media were supplemented with 10% fetal bovine serum, penicillin/streptomycin, and L-glutamine.
8
Comparative Analysis of Renal Cell Carcinoma Cell Lines
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A-704 (ATCC® HTB-45™), 786-O (ATCC® CRL-1932™) , ACHN (ATCC® CRL-1611™), Caki-1 (ATCC® HTB-46™) , HEK293 (ATCC® CRL-1573TM) , A498 (ATCC® HTB-44™) , HREC ( ATCC® PCS-400-012) and HUVEC (ATCC® CRL-1730™) cells were all obtained from ATCC. ACHN, A704 and HEK293 cells were cultured using EMEM (Eagle’s minimal essential medium). HUVEC cells were grown with F-12K Medium with Heparin and Endothelial cell growth supplement. 786-O and A498 cells were cultured with RPMI 1640 Media. Caki-1 cells were cultured with McCoy’s 5A Modified Medium. All cell media was supplemented with 10% Fetal Bovine Serum and 1X penicillin/streptomycin. For all transfections, antibiotics were excluded from media. Cells were grown and maintained at 37°C, 5% CO2. Frozen cultures were stored at −80C or long-term in liquid nitrogen.
9
Characterization of mccRCC Tumor Samples
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De-identified mccRCC tissue samples were obtained from patients after receiving informed consent in Vancouver General Hospital (H09–01628). Primary kidney tumor specimens from mccRCC patients with or without sunitinib treatment were considered for further analysis. Each group had more than 5 patient samples. Caki-1 (ATCC, VA, USA) was grown in McCoy’s 5A media (Gibco, MD, USA) supplemented with 10%FBS (Hyclone, UT, USA). 786-O (ATCC, VA, USA) was grown in RPMI media (Gibco, MD, USA) supplemented with 10%FBS (Hyclone, UT, USA). Human Umbilical Vein Endothelial Cells (HUVEC) from pooled donors (Lonza, GA, USA) were maintained in EBM-Plus Bulletkit (Lonza, GA, USA). Cells were passaged 0.25% Trypsin-EDTA (Gibco, MD, USA). Where appropriate, cell numbers were counted with Automated Cell Counter TC20 (Bio-Rad, WA, USA). All cells were incubated at 37 °C in 5% CO2.
10
Human Kidney Cancer Cell Lines
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