Ethanolamide

Detergents (sodium dodecyl sulfate (SDS) and Triton X-100), dithiothreitol (DTT), glycerol, penicillin, streptomycin, Schneider's Drosophila medium, protein A-agarose, transepoxysuccinyl-L-leucylamido(4-guanidino)butane (E-64), the silver Stain kit Proteo Silver™, nitrocellulose membranes, and cell culture flasks were purchased from Sigma-Aldrich Chemical Co. (St. Louis, MO). The micro-bicinchoninic acid (BCA) protein assay kit was purchased from Pierce Chemical Co. (Appleton, WI). Fetal calf serum (FCS) was acquired from Gibco, Invitrogen (Brazil). A bacterial expression vector containing the T7lac promoter (pET28a) was purchased from GenScript, Inc. (Piscataway, NJ, USA). Nickel-charged (Ni-NTA) resin was obtained from Qiagen (Brazil). The full-range rainbow™ kit, 12 to 225 kDa, was purchased from GE Healthcare Life's Sciences (Little Chalfont, UK). Coupling agents [1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) and N-hydroxysuccinimide (NHS)] and ethanolamide were purchased from Merck Millipore Corporation (Darmstadt, Alemanha). A carboxylated gold sensor chip (COOH1 chip) was acquired from ICx Nomadics, Inc. (Stillwater, OK). Chemiluminescence was performed using the SuperSignal West Pico Chemiluminescent kit™ Substrate (Thermo Scientific, EUA). All reagents were of analytical grade or higher.