Gammacell 40 irradiator chamber

A total of 1,000,000 virally infected murine hematopoietic stem/ progenitor cells cultured in erythroid maintenance medium, produced as described above, were collected and resuspended in 100μl PBS. These cells were then injected retro-orbitally into a C57BL/6J (Jackson Laboratory) mouse irradiated at 1050 rad, 1 day prior to transfer, in a Gammacell 40 irradiator chamber (Nordion International Inc.), and transplanted mice were monitored for 4 weeks before further analyses.

C57BL/6J (Jackson Laboratory) or CD-1 (Charles River) mice were subjected to lethal irradiation of 1050 rad carried out in a Gammacell 40 irradiator chamber (Nordion International Inc.) 1 day before transplantation. A total of 1,500,000 virally infected murine erythroid progenitors from mouse fetal liver cultured in erythroid maintenance medium, produced as described in the previous paragraph, were harvested and resuspended in 100 μl PBS^{32 (link)}. These cells were then injected retro-orbitally into an irradiated mouse. Mice were bled for examining chimeric protein expression and further analysis 5 weeks after transplantation. Mice were bled at the indicated time points for performing CBC analysis on a SIEMENS ADVIA 2120i machine.