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Mda mb 231 mm231

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MDA-MB-231 (MM231) is a cell line derived from a human breast adenocarcinoma. It is a widely used model for cancer research.

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Lab products found in correlation

Skbr3, by American Type Culture Collection (1 mentions) Sk br 3, by Thermo Fisher Scientific (1 mentions) Non essential amino acids (neaa), by Thermo Fisher Scientific (1 mentions) Insulin, by Merck Group (1 mentions) Mcf 7, by Thermo Fisher Scientific (1 mentions) Mycoalert mycoplasma detection kit, by Lonza (1 mentions) Mda mb 468 mm 468, by American Type Culture Collection (1 mentions)

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MDA-MB-231 (5102 citations) MDA-231 (69 citations)

2 protocols using mda mb 231 mm231

1

Maintenance of Breast Cancer Cell Lines

Cited 2 times
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MDA-MB-134-VI (MM134), MDA-MB-330 (MM330), MCF7, T47D, MDA-MB-231 (MM231) and SKBR3 cells were obtained from the American Type Culture Collection. SUM44PE (SUM44) cells were purchased from Asterand and BCK4 cells were kindly provided by Britta Jacobsen, University of Colorado Anschutz, CO. Cell lines were maintained as previously described25 (link) in the following media (Life Technologies) with 10% FBS: MM134 and MM330 in 1:1 DMEM:L-15, MCF7 and MM231 in DMEM, T47D in RPMI, SKBR3 in McCoy’s 5A, BCK4 in MEM with non-essential amino acids (Life Technologies) and insulin (Sigma-Aldrich). SUM44 cells were maintained as described61 (link) in DMEM-F12 with 2% charcoal stripped serum and supplements. Cell lines were routinely tested to be mycoplasma free using MycoAlert Mycoplasma Detection Kit (Lonza; #LT07-418), authenticated by the University of Arizona Genetics Core by Short Tandem Repeat DNA profiling and kept in continuous culture for < 6 months.
Tasdemir N., Ding K., Savariau L., Levine K.M., Du T., Elangovan A., Bossart E.A., Lee A.V., Davidson N.E, & Oesterreich S. (2020). Proteomic and transcriptomic profiling identifies mediators of anchorage-independent growth and roles of inhibitor of differentiation proteins in invasive lobular carcinoma. Scientific Reports, 10, 11487.
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2

Culturing Triple-Negative Breast Cancer Cells

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Triple-negative breast cancer cells (MDA-MB-231 (MM-231) (Caucasian) and MDA-MB-468 (MM-468) (African American)) were purchased from American Type Culture Collection (ATCC) (Manassas, VA, USA). The cells were grown in 75 mm flasks using DMEM, 10% heat inactivated fetal bovine serum (FBS-HI) and 1% penicillin/streptomycin (100 U/ml penicillin and 0.1 mg/ml streptomycin). Cell cultures were incubated in an atmosphere of 5% CO2 at 37°C. Experimental media consisted of DMEM supplemented with 2.5% of FBS-HI.
MENDONCA P., DARWISH A.G., TSOLOVA V., EL-SHARKAWY I, & SOLIMAN K.F. (2019). The Anticancer and Antioxidant Effects of Muscadine Grape Extracts on Racially Different Triple-negative Breast Cancer Cells. Anticancer research, 39(8), 4043-4053.
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