Ad shctrl

Ad-Slc40a1 and Ad-Ctrl constructs were generated by Hanbio Biotechnology (China) using adenoviral vectors carrying Slc40a1. Ad-shSlc40a1 and Ad-shCtrl were provided by GeneChem Technology (Shanghai, China), in the form of adenoviral vectors carrying shRNA-Slc40a1.Protein expression was observed 48 hours after viral infection. The plasmid containing Steap4 and the negative control plasmid (OE-NC) were created and produced by Tsingke Biotech (China). Cardiomyocytes were transfected with the plasmid using Lipofectamine 3000 from Invitrogen (USA). Protein expression was measured 72 hours after transfection. The shRNA target sequences are shown in Table S3.

The CCl₄ and TGF-β1 were purchased from Sigma (St Louis, MO, USA). Antibodies against type I collagen (Affinity, NO. AF7001, 1:1000), α-SMA (Abcam, NO. ab32575, 1:2000), E-cadherin (Sigma, NO. MABT26, 1:1000), BMP-7 (Sigma, NO. MAB4350, 1:1000), Desmin (Proteintech, NO. 16520-1-AP, 1:50000), Vimentin (Proteintech, NO. 60330-1-Ig, 1: 50000), YAP (Proteintech, NO. 13584-1-AP, 1: 5000), TAZ (Proteintech, NO. 23306-1-AP, 1:2000), p-YAP (Affinity, NO. AF3328, 1:1000), p-TAZ (Affinity, NO. AF4315, 1:1000), MST1 (Affinity, NO. DF8430, 1:2000), p-MST1 (Affinity, NO. AF3688, 1:1000), LATS1 (Affinity, NO. AF7669, 1:1000), p-LATS1 (Affinity, NO. AF7169, 1:1000), NF2 (Abcam, NO. ab109244, 1:100000) and GAPDH (Abcam, NO. ab9485, 1:2500) were purchased. Adenoviral vectors expressing the scrambled shRNA (Ad-shCtrl), Ad-shSNHG5, adenoviral vectors expressing a control scrambled sequence (Ad-Ctrl), Ad-SNHG5 and adenoviral vectors expressing NF2 (Ad-NF2) were purchased from Genechem Co.Ltd (Shanghai, China). Animal experimental protocols were approved by the University Animal Care and Use Committee after mice were supplied by the Experimental Animal Center of Wenzhou Medical University.