Trans well plates and inserts

All chemicals, cell culture media, and supplements for cell culture and submerged exposure were purchased from Gibco/Thermo Fisher Scientific GmBH (Dreieich, Germany), and all chemicals for ALI exposure were obtained from Sigma-Aldrich Chemie GmBH (Taufkirchen, Germany) or Carl Roth GmbH (Karlsruhe, Germany), except for fetal bovine serum (FBS), which was obtained from Thermo Fisher Scientific GmBH (Dreieich, Germany). Cell culture consumables such as plates, flasks, and centrifuge tubes were purchased from Sarstedt (Nuembrecht, Germany) and Greiner (Kremsmünster, Germany). Trans-well plates and inserts were bought from Corning (Amsterdam, The Netherlands). Min-U-Sil5 quartz particles were purchased from U.S. Silica (Katy, TX, USA). Endotoxin contamination was measured via LAL Chromogenic Endotoxin Quantitation Kit (ThermoFisher Scientific, Karlsruhe, Germany). The LDH assay kits were acquired from Promega (Madison, WI, USA) and Roche (Mannheim, Germany). Alamar Blue was obtained from AbD Serotec (Purchheim, Germany). IL-8 ELISAs were purchased from Invitrogen (Waltham, MA, USA).

All chemicals, media, and supplements for cell culture and ALI exposure were purchased from Gibco/Thermo Fisher Scientific GmBH (Dreieich, Germany) and Roth (Karlsruhe, Germany), except for KGM, which was acquired from Lonza (Basel, Switzerland). The PAN-based CF Tenax^®-J UMS40 was purchased from Teijin Carbon Europe GmbH (Wuppertal, Germany). Cell culture consumables were obtained from Greiner (Kremsmünster, Germany), and Transwell^® plates and inserts were produced and purchased from Corning (Amsterdam, The Netherlands). LDH assay kits were bought from Roche (Mannheim, Germany). Triton X-100 was purchased from Roth (Karlsruhe, Germany). Primers for RT-qPCR were synthesized by Eurofins (Ebersberg, Germany). PCR reagents were acquired from Macherey-Nagel (Dueren, Germany), Applied Biosystems (Foster City, CA, USA), Teknova (Hollister, OH, USA), Fluidigm (San Francisco, CA, USA), Bio-Rad (Munich, Germany), and New England Biolabs (Frankfurt am Main, Germany). IL-8 ELISA kits were obtained from Invitrogen (Waltham, MA. USA). LPS and Menadione were purchased from Sigma-Aldrich (Steinheim, Germany).

All chemicals, cell culture media, and supplements were obtained from Sigma–Aldrich Chemie GmBH (Taufkirchen, Germany) or Carl Roth GmbH (Karlsruhe, Germany), except for fetal bovine serum (FBS), which was obtained from Thermo Fisher Scientific GmBH (Dreieich, Germany). Transwell plates and inserts were acquired from Corning (Amsterdam, Netherlands). Other cell culture materials, such as dishes, flasks, and reaction tubes were bought from Sarstedt (Nuembrecht, Germany). Min-U-Sil5 particles were purchased from U.S.Silica (Katy, TX, USA). Endotoxin content was assessed with the LAL Chromogenic Endotoxin Quantitation Kit (ThermoFisher Scientific, Karlsruhe, Germany). Primers for RT-qPCR were synthesized by Eurofins (Ebersberg, Germany). PCR reagents were purchased from Macherey–Nagel (Dueren, Germany), Applied Biosystems (Foster City, WI, USA), Teknova (Hollister, CA, USA), Fluidigm (San Francisco, CA, USA), Bio-Rad (Munich, Germany) and New England Biolabs (Frankfurt am Main, Germany).