Nicolet avatar 370 ft ir spectrometer

All reagents, including benzonitrile, 2-cyanophenol, NH₄Cl, 3-cyanophenol, 4-cyanophenol, DMF, anhydrous K₂CO₃, ethyl acetate, petroleum ether, and sodium hydroxide, were purchased from Aladdin (Shanghai, China) and were of analytical grade. Sodium azide, of an analytical grade, was purchased from Tianjin Fengchuan Chemical Reagent Technology Co., Ltd. (Tianjin, China). A total of 36–38% hydrochloric acid, acetic anhydride, methanol, butanone, dichloromethane, and acetone were analytical graded and provided by Beijing Tongguang Fine Chemicals Co., Ltd. (Beijing, China). Potassium bromide was of chromatographic grade and purchased from China National Pharmaceutical Group Chemical Reagent Co., Ltd. (Beijing, China). All reagents were used as received without further purification. Infrared (IR) spectra were acquired using a Thermo Nicolet AVA-TAR 370 FT-IR spectrometer. ¹H NMR and ¹³C NMR analyses were performed on an Advance Bruker III 400 (400 MHz) NMR spectrometer, with TMS as the internal standard and DMSO-d₆ as the solvent. UV-vis spectroscopy was carried out using a Hitachi U4150 ultraviolet spectrometer in the 200–600 nm range, with methanol were used as the solvent for sample analysis. The separation of compounds was accomplished by High-Resolution Mass Spectrometry (HRMS) utilizing a Triple Quadrupole MS instrument (Waters India Pvt Ltd., Karnataka, India).

Scanning electron microscope (SEM) images were taken on a Shimadzu SSX-550 field emission scanning electron microscope (Shimadzu, Co. Ltd., Kyoto, Japan) at 15.0 kV. The as-electrospun samples were dispersed in ethanol with the assistance of an ice-water ultrasonic bath at 35 kHz, and a drop of the well-dispersed sample was cast repeatedly onto a piece of silicon wafer and air-dried. A thin gold coating was utilized to avoid charging during scanning, and a thorough microscopic study was carried out. The detailed morphology and size were examined with a Hitachi H-8100 transmission electron microscope (TEM, Hitachi, Ltd., Tokyo, Japan). As for SEM measurement, the as-electrospun samples for TEM were dispersed in ethanol with the assistance of an ice-water ultrasonic bath at 35 kHz. A drop of the well-dispersed sample above was cast repeatedly onto a specimen holder and air-dried. Fourier transform infrared (FTIR) spectra were recorded on a Thermo Nicolet Avatar 370 FTIR spectrometer. UV–visible absorption spectra (UV–vis, Hitachi, Ltd., Tokyo, Japan) were collected with a Hitachi U-3900H spectrophotometer in the 300–700 nm wavelength range. X-ray photoelectron spectra (XPS) were recorded with Mg Kα radiation.