A2780 and Caov-3 cell proliferation after 48 of treatment with pleuromutilin was determined using Edu proliferation assay in accordance with the manufacturer’s instructions. The cells were distributed at 3×104 cells/well density in 96-well plates and cultured for 24 h. The medium was changed by fresh medium mixed with 10, 20, 40, 80, 160, and 200 μM of pleuromutilin, and incubation was performed for 48 h. Cell proliferation was determined using an Edu proliferation assay kit (Guangzhou RiboBio Co., Ltd., Guangzhou, China). An Olympus IX51 fluorescence microscope (Olympus Corporation, Tokyo, Japan) was used to observe the stained cells.
Edu proliferation assay kit
Manufactured by RiboBio
Sourced in China
The EdU proliferation assay kit is a laboratory tool used to detect and quantify cell proliferation. It functions by incorporating the nucleoside analogue EdU (5-ethynyl-2'-deoxyuridine) into the DNA of dividing cells, which can then be detected using a fluorescent dye. This allows for the visualization and measurement of cell proliferation.
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Lab products found in correlation
4 protocols using edu proliferation assay kit
1
Pleuromutilin Inhibits A2780 and Caov-3 Cell Proliferation
2
Corilagin Modulates Cell Proliferation
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The cells (density, 1×104 cells/well) were seeded into a 96-well plate and then treated with corilagin for 24 h. Cell proliferation was assessed using the EdU proliferation assay kit (Guangzhou RiboBio Co., Ltd., Guangzhou, China). The stained cells were observed under a fluorescence microscope (Olympus IX51; Olympus Corporation, Tokyo, Japan).
3
Wogonin Cytotoxicity in A549, A427, BEAS-2B Cells
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The A549, A427, and BEAS-2B cells at 2x104 cells/well density were distributed in 96 well plates. The cells were treated with 5, 10, 15, 20, 25, 30, and 50 µM of wogonin for 72 h. The changes in cell proliferation were determined by EdU proliferation assay kit (Guangzhou RiboBio Co., Ltd., Guangzhou, China). Fluorescence microscope (Olympus IX51; Olympus Corporation, Tokyo, Japan) was used for observing the EdU stained cells.
4
Cell Proliferation Tracking via EdU Assay
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Cell proliferation was detected by EdU proliferation assay kit (RiboBio, China). Cells that underwent transfection were placed onto glass slides within 12-well culture dishes. Once the cells reached a 40% abundance, a low concentration of EDU solution was introduced and left to incubate for 24 hours. Following the manufacturer's instructions, the cells were first treated with 4% paraformaldehyde for fixation, then permeabilized with Triton, and finally stained with Cy3 and DAPI. Photographs were then taken with a fluorescence microscope (Olympus, Japan).
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