Advance av500 spectrometer

Infrared radiation spectra were recorded with a Thermo Scientific Nicolet iS5 FT-IR spectrometer (Thermo, Waltham, MA, USA). Nuclear magnetic resonance spectra were measured with a Bruker Advance AV500 spectrometer (Bruker, Mannheim, Germany). LC-MS was performed with an Agilent 1290 system equipped with a 6120 Quadrupole MSD mass spectrometer (Agilent Technologies, Santa Clara, CA, USA). HRESIMS spectra were recorded with a AB Sciex TripleTOF 5600 mass spectrometer (AB Sciex, Redwood City, CA, USA). HPLC analyses were conducted on an Agilent 1260 system with a PDA detector (Agilent Technologies, USA) and a Waters 2695 system with a 2998 PDA detector (Waters, Milford, MA, USA). Preparative HPLC separation was conducted on a Waters 1525EF LC system (Waters). MCI Gel high-porous polymer (75–150 μm) was obtained from Mitsubishi Chemical Corporation (Mitsubishi, Sagamihara, Japan). Sephadex LH-20 resin (25–100 μm) was obtained from GE Healthcare Company (GE Healthcare, Danderyd, Sweden). Amberlite XAD16N resin was purchased from Taitan Company (Shanghai, China). Chemicals were purchased from Aldrich or Juyou Company (Nanjing, China).

UV data were acquired on a Persee TU-1810 spectrophotometer (Persee analytics, Beijing, China). IR spectra were measured on a Thermo Scientific Nicolet iS5 FT-IR spectrometer (Thermo, United States). NMR spectra were obtained on a Bruker Advance AV500 spectrometer (Bruker, Germany). HRESIMS spectra were recorded on an Orbitrap Elite mass spectrometer (Thermo Scientific, United States). Liquid chromatography–mass spectrometry (LC-MS) was conducted with an Agilent 1290 system equipped with 6120 Quadrupole MSD mass spectrometer (Agilent Technologies, United States). HPLC analysis was performed on a Waters 2695 system equipped with 2998 PDA detector. Total component analysis was performed on an Agilent 1290 UHPLC-6520 Q-TOF/MS. Preparative HPLC separation was performed on a Waters 1525 EF LC system (Waters Company, United States). MCI GEL high-porous polymer (75–150 μm) was purchased from Mitsubishi Chemical Corporation (Japan). Sephadex LH-20 resin (25–100 μm) was purchased from GE Healthcare Company (Sweden). XAD16N resin (20–60 mesh) was obtained from Yuanye Company (Nanjing, Jiangsu, China). Chemicals were purchased from Juyou Company or Aldrich and used without further purification unless otherwise noted.