Ab3080

Manufactured by Abcam

AB3080 is a lab equipment product. It is a core functional component used in scientific research and analysis. The detailed specifications and intended use of this product are not available at this time.

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Lab products found in correlation

Ab76493, by Abcam (1 mentions) Goat anti rabbit igg h l fitc, by Abcam (1 mentions) 4 6 diamidino 2 phenylindole dihydrochloride dapi, by Thermo Fisher Scientific (1 mentions) Goat anti mouse igg h l cy3, by Abcam (1 mentions) Anti flag m2 affinity gel, by Merck Group (1 mentions) α tubulin, by Merck Group (1 mentions) Ni nta resin, by Qiagen (1 mentions) Ab51502, by Abcam (1 mentions) Ab23345, by Abcam (1 mentions) Ab31940, by Santa Cruz Biotechnology (1 mentions) Ab18465, by Abcam (1 mentions) Hoechst 33258, by Thermo Fisher Scientific (1 mentions) Vt100, by Leica camera (1 mentions) Sc 13024, by Santa Cruz Biotechnology (1 mentions) Sc 19233, by Santa Cruz Biotechnology (1 mentions)

3 protocols using ab3080

Antibody Validation for Protein Interactions

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Antibodies against HSPD1 (mouse original, ab3080), IRF3 (rabbit original, ab76367), and phospho S386-IRF3 (rabbit original, ab76493) were purchased from Abcam, HK. Anti-myc McAb (rabbit original, 9402S), anti-flag McAb (rabbit original, 2368S) were from Cell Signaling Technology. Anti-Flag antibody (M2, mouse) and the ANTI-FLAG M2 Affinity Gel were from Sigma. Anti-GAPDH was from Cali-Bio (mouse original, CB100127). The secondary antibodies goat anti-rabbit Fc-HRP 4041-05 and goat anti-mouse Fc-HRP 1033-05 were from SouthernBiotech. The secondary fluorescence-labeled antibodies goat anti-rabbit IgG H&L (FITC, ab6717) and goat anti-mouse IgG H&L (Cy3, ab97035) were from Abcam. The 4–6-diamidino-2-phenylindole-dihydrochloride (DAPI) was from Invitrogen.

Lin L., Pan S., Zhao J., Liu C., Wang P., Fu L., Xu X., Jin M, & Zhang A. (2014). HSPD1 Interacts with IRF3 to Facilitate Interferon-Beta Induction. PLoS ONE, 9(12), e114874.

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Xenopus Xili Gene Model Characterization

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The Xenopus Xili gene model was identified in the Ensembl v52 database for X. tropicalis (model ENSXETG00000010533). Amino acids 1–300 were amplified by PCR from Xenopus oocyte cDNA, and cloned into pET30a (pMB192). Protein was expressed at 22°C and purified using Ni–NTA resin (QIAGEN). Recombinant XIWI was used to generate antibodies in a rabbit (Covance), as described in Lau et al. (2009a) (link). Laboratory antibodies were affinity purified before use. Other antibodies used in this study were α-tubulin (SIGMA T9026), trap-α (Covance, custom rabbit), and hsp60 (Abcam AB3080).

Toombs J.A., Sytnikova Y.A., Chirn G.W., Ang I., Lau N.C, & Blower M.D. (2017). Xenopus Piwi proteins interact with a broad proportion of the oocyte transcriptome. RNA, 23(4), 504-520.

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Immunohistochemical Analysis of Embryonic and Postnatal Brains

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Pregnant females or postnatal animals were euthanized by lethal intreperitoneal injection of pentobarbital (50 mg/kg). Brains from embryos were dissected and fixed overnight (O.N.) in cold paraformaldehyde (PFA, 4%, pH 7.4). For postnatal brains, animals were perfused with intracardial 0.9% saline followed by cold 4% PFA. Brains were cut on a Vibratome (Leica VT100S) for immunohistochemistry (IHC). Sections were kept at 4°C in 0.1 M phosphate buffer saline (PBS) and were stained as described (Riccio et al., 2011 (link)) with the following primary antibodies: goat anti-GFP (1/500, Abcam, ab5450), goat anti-GFP (1/1000, Millipore, AB3080), rabbit anti-5-HT6R (1/500, Abcam, ab103016), rabbit anti-CUX1 (1/250, Santa Cruz, sc-13024), rabbit anti-TLE4 (1/500, Santa Cruz, sc-9125), rat anti-CTIP2 (1/500, Abcam, ab18465), rabbit anti-TBR2 (1/500, Abcam, ab23345), rabbit anti-TBR1 (1/500, Abcam, ab31940), goat anti-Ngn2 (1/50, Santa-Cruz, sc-19233), goat anti-Brn2 (1/50, Santa-Cruz, sc-6029), mouse anti-SATB2 (1/500, Abcam, ab51502), secondary goat or donkey Alexa-488, -568 and -647 antibodies (Molecular Probes, Invitrogen) raised against the appropriate species were used at a dilution of 1/1000 and sections were counterstained with Hoechst 33258 (1/10,000, Life Technologies, H3569).

Jacobshagen M., Niquille M., Chaumont-Dubel S., Marin P, & Dayer A. (2014). The serotonin 6 receptor controls neuronal migration during corticogenesis via a ligand-independent Cdk5-dependent mechanism. Development (Cambridge, England), 141(17), 3370-3377.

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