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Snu 333

Manufactured by Korean Cell Line Bank

SNU-333 is a cell culture incubator designed for the maintenance and growth of cell lines. It provides a controlled environment with adjustable temperature, humidity, and CO2 levels to support the optimal conditions for cell culture. The core function of SNU-333 is to provide a stable and consistent environment for the cultivation of various cell types.

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2 protocols using snu 333

1

Cell Line Cultivation and Sourcing

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MDA-MB-231, HCT-116, AGS, SK-MEL-2, SNU-1214, SNU-333, SNU-475, SW620, and HeLa cells were obtained from Korean Cell Line Bank (Seoul, Korea). U-2-OS, A549, and H1975 cells were obtained from the American Type Culture Collection (Manassas, Virginia, USA). HeLa, U-2-OS, A549, and NCI-H1975 cells were grown in Dulbecco’s Modified Eagle Medium; all other cell lines were grown in Roswell Park Memorial Institute Medium (RPMI)-1640 supplemented with 10% fetal bovine serum.
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2

Culturing Kidney and Renal Cancer Cells

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Human kidney proximal tubule cells (HK-2) and RCC cells (Caki-1, Caki-2, SNU-333, SNU-349, and SNU-1272) were purchased from the Korean Cell Line Bank, and cultured according to the supplier’s protocol. HK-2, SNU-333, SNU-349, and SNU-1272 cells were cultured in RPMI-1640 medium, and Caki-1 and Caki-2 cells were cultured in DMEM/high glucose. All media were from Welgene and contained 10% fetal bovine serum and 1% penicillin-streptomycin. The cells were grown in monolayers at 37 °C in a 5% CO2 incubator unless otherwise described.
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