The migration of lung cancer A549 cell lines was tested using Transwell chamber (8 µm pore; Corning, Inc., Corning, NY, USA). Approximately 1×106 cells were added into the upper chamber and cell medium was added into the lower chamber. Cells were incubated for 24 h at 37°C. Crystal violet was used to stain the migrated cells, which were visualized using an inverted microscope. The upper chamber was filled with Matrigel (BD Biosciences, San Jose, CA, USA) for the invasion test.
Transwell chamber 8 m pore
Manufactured by Corning
Sourced in United States
The Transwell chamber (8 µm pore) is a laboratory equipment used for cell culture and migration studies. It consists of a membrane with 8 micrometer pores that separates an upper and lower compartment, allowing for the control and observation of cellular interactions and movement across the membrane.
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2 protocols using transwell chamber 8 m pore
1
Transwell Assay for Cell Migration and Invasion
2
Transwell Assay for Cell Migration
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The migratory potential was measured using a Transwell chamber (8-µm pore, Corning). Two hundred microliters of a suspension containing 80,000 transfected cells was plated in medium with 1% FBS in the top chamber of a transwell apparatus, while seven hundred microliters of medium containing 20% FBS was placed in the lower well. After 24 h of incubation, cells were fixed in 4% paraformaldehyde for 15 min and dyed with 0.1% crystal violet for 20 min. Cells in five fields in each well were captured under a microscope (magnification ×200).
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