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2 protocols using 3 methacryloxypropyl trimethoxy silane

1

Synthesis of Silica-Based Polymer Nanoparticles

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Tetraethoxysilane (TEOS, ≥99%), styrene (≥99%, with ca. 50 ppm 4-tert-butylcatechol as stabilizer), sodium persulfate (Na2S2O8, ≥99%), polyethylene glycol nonylphenyl ether (Synperonic® NP30), sodium dodecylsulfate (SDS, 99%), hydroquinone (≥99%), cyclohexane (≥99.7%) and tetrahydrofuran (THF, ≥99% contains 250 ppm butylhydroxytoluene as inhibitor) were purchased from Sigma-Aldrich (Saint-Quentin Fallavier, France). 3-methacryloxypropyl(trimethoxy)silane (MPS, 98%) was purchased from ABCR (Karlsruhe, Germany). Ammonium hydroxide (NH4OH, 28–30% in water) and ethanol (99%) were provided by Atlantic Labo (Bruges, France). Oleic acid (65–88%) was purchased from Fisher Scientific (Illkirch-Graffenstaden, France). Deionized water with a resistivity of 18.2 MΩ·cm at 25 °C was obtained from a Milli-Q system (Merck Millipore, Darmstadt, Germany). All chemicals were used without further purification.
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2

Biofunctionalized Silane Coatings Synthesis

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Methyltrimethoxysilane (98% purity, MTMS), (3-mercaptopropyl)trimethoxysilane (95% purity, MPTMS), hexadecyltrimethylammonium bromide (98% purity, CTAB), methanol (99.8%, MeOH) and tetrakis(acetonitrile)copper(I) hexafluorophosphate were acquired from Sigma Aldrich. Oxalic acid, vinyltrimethoxysilane (97% purity, VTMS), Ellman's reagent (5,5′-dithiobis-(2-nitrobenzoic acid) or DTNB) and sodium azide (NaN 3 ) were from Merck. (3-Chloropropyl)-trimethoxysilane (97% purity; CPTMS) and (3-methacryloxypropyl)trimethoxysilane (98% purity, MAPTMS) were purchased from ABCR. Propiolic acid (98%) was obtained from Acros Organics. Ammonium hydroxide (28% in H 2 O), sodium carbonate (Na 2 CO 3 ), potassium permanganate (KMnO 4 ), N,N-dimethylformamide (DMF), acetonitrile (ACN) and sodium azide were procured from VWR.
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