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Magnesium chloride (MgCl2) is a chemical compound that serves as a key laboratory reagent. It is a crystalline solid that readily dissolves in water and other polar solvents. The primary function of MgCl2 is to provide a source of magnesium ions (Mg2+) in various experimental and analytical procedures.

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7 protocols using mgcl2

1

Anthraquinone-Based Metal Complexes Synthesis

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1,4-dihydroxyanthraquinone, CoCl2, NaCl, CuSO4, ZnCl2, MgCl2, HgCl2, Ni(NO3)2, MnCl2, NH4Cl, Al2(SO4)3, CoCl2, formamide, ethanol, dichloromethane (DCM), glyphosate (GP), trichlorfon, 1-naphthofen, carbaryl, carbendazim, polyvinyl alcohol (PVA), acrylamide, ammonium persulphateetc, and all of the reagents were purchased from Shanghai Macklin Biochemical Co., Ltd (http://www.macklin.cn/) and were used without further treatment.
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2

Biomimetic Hydrogel Synthesis and Characterization

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Benzyl methacrylate (BzMA, 98%, Energy Chemical), ethyl methacrylate (EMA, 99%, Aladdin), 2-hydroxyethyl methacrylate (HEMA, 99%, Aladdin), dimethylacrylamide (DMAA, 98%, Energy Chemical), 1-ethyl-3-methyl imidazolium bis(trifluorosufonylmethane imide) (EMITFSI, 99%, Lanzhou Greenchem), 1-butyl-3-methyl imidazolium hexafluorophosphate (BMIPF6, 99%, Lanzhou Greenchem), divinylbenzene (DVB, 80%, Energy Chemical), 1-hydroxycyclohexyl phenyl ketone (PI-184, 98%, Aladdin), N,N-dimethylformamide (DMF, 99.9%, Energy Chemical), tetrahydrofuran (THF, 99.9%, Energy Chemical), sodium chloride (NaCl, 99.9%, Energy Chemical), calcium chloride (CaCl2, 97%, Sigma-Aldrich), magnesium chloride (MgCl2, 99%, Macklin), potassium chloride (KCl, 99.5%, Energy Chemical), sodium bicarbonate (NaHCO3, 99.5%, SCR) and magnesium sulfate (MgSO4, 99.9%, Energy Chemical) were used as received.
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3

Oligonucleotide-Based Electrochemical Biosensor

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All oligonucleotides used in this study were obtained from Sangon Biotech Co., Ltd. (Shanghai, China). The oligonucleotide sequences are described in Table 1. NH4F, MgCl2, and ethylene diamine tetraacetic acid (EDTA) were procured from Shanghai Macklin Biochemical Co., Ltd. (Shanghai, China). HAuCl4·4H2O was obtained from Kema Biochemical (Tianjin, China). 6-Mercapto-1-hexanol (MCH), tris (2-carboxyethy) phosphine hydrochloride (TCEP), and tris (hydroxymethyl) methyl aminomethane (Tris-base) were obtained from Sigma–Aldrich (St. Louis, USA). Ni(NO3)2·6H2O, Fe(NO3)3·9H2O, CO(NH2)2, Na2HPO4, and NaH2PO4 and NaCl were procured from Guangzhou Chemical Reagent Factory (Guangzhou, China). SnO2 transparent conductive glass (FTO, 1 cm × 2 cm) was purchased from Yingkou New Energy Technology (Yingkou, China). Double distilled water was utilized during the experiment. All chemicals were of analytical reagent grade and used directly after purchase.
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4

Naringenin Metabolism in Rat Liver Microsomes

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Naringenin standard was purchased from Chengdu Must-Technology Co., Ltd. (Chengdu, China). Its purity was acceptable (≥98%) according to HPLC–UV analysis, and its structure was fully elucidated by comparing the spectral data (ESI-MS and 1H, 13C-NMR spectroscopy) with the literature. HPLC-grade acetonitrile, methanol, and formic acid (FA) were purchased from Thermo Fisher Scientific (Fair Lawn, NJ, USA). The deionized water used throughout the experiment was purchased from Watsons (Guangzhou, China). Oasis® HLB C18-low solid-phase extraction cartridges (500 mg·6 mL−1, 60 µm, 149 Å) were purchased from Waters Corporation (Milford, MA, USA). Rat liver microsomes were obtained from NEWGAINBIO Co., Ltd. (Wuxi, China). Nicotinamide adenine dinucleotide phosphate (NADPH) and MgCl2 were purchased from Shanghai Macklin Biochemical Co., Ltd. (Shanghai, China). Six-well plates were obtained from Corning Incorporated-Life Science (Hangzhou, China).
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5

Alkaline Lignin Valorization Protocol

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The alkaline lignin was provided by Shandong Longlive Bio-Technology Co., Ltd., China, a by-product of xylooligosaccharides production from corn cob. The lignin was composed of 90.81% Klason lignin, 3.61% acid-soluble lignin, 0.63% sugars, 2.16% ash, and 2.79% others (Shao et al., 2018 (link)). 5 wt% Ru/C, formic acid (FA, 99%), ethyl acetate (HPLC grade, 99.9%), and acetophenone (standard for GC, 99.5%) were provided by Aladdin® company. Methanol (99.5%), ethyl acetate (99.5%), and tetrahydrofuran (THF, 99.0%) were analytical grade and provided by Beijing Chemical Works. AlCl3, CrCl3, LiCl, NaCl, FeCl3, FeCl2, MgCl2, ZnCl2, KCl, and Zn(OAC)2 were also analytical grade and purchased from Macklin® company. All reagents were used as received.
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6

Pterostilbene Metabolism in Rat Liver

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Pterostilbene
(purity ≥99.99%, MUST-21052207) was provided by Chengdu Must
Biotechnology Co. Ltd. (Sichuan, China); HPLC-grade acetonitrile,
methanol, and formic acid were purchased from Thermo Fisher Scientific
(Fair Lawn, NJ, USA). The deionized water used throughout the experiment
was purchased from Watsons (Guangzhou, China). Rat liver microsomes
were obtained from Xin Run Biotechnology Co., Ltd. (Wuxi, China);
Oasis HLB C18-Low solid-phase extraction (SPE) cartridges (500 mg/6
mL, 60 μm, 149 Å) were purchased from Waters Corporation
(Milford, USA). Nicotinamide adenine dinucleotide phosphate (NADPH)
and MgCl2 were purchased from Shanghai Macklin Biochemical
Co., Ltd. (Shanghai, China). Six-well plates were obtained from Corning
Incorporated-Life Science (Jiangsu, China).
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7

Characterization of Honey Samples

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Terbium chloride hexahydrate (TbCl3·6H2O), europium chloride hexahydrate (EuCl3·6H2O), glucose, fructose, vitamin B6, vitamin B2, vitamin C, kaempferol, quercetin, caffeic acid, p-coumaric acid, and vanillic acid were obtained from Aladdin (Shanghai, China). Dipicolinic acid (DPA), proline, gluconic acid, citric acid, succinic acid, MgCl2, ZnCl2, CuCl2, and MnCl2 were obtained from Macklin (Shanghai, China). Other reagents were all of analytical grade. Honey samples were directly collected from apiaries during the period of flowering. Litchi (Litchi chinensis) and longan (Dimocarpus longan) honey samples were collected in Fujian province, and astragalus (Astragalus membranaceus) honey samples were collected in Gansu province. Ultrapure water (18.2 MΩ·cm) was used in all the experiments.
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