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Phospho rac1 cdc42

Manufactured by Cell Signaling Technology
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Phospho-Rac1/cdc42 is a lab equipment product that detects the phosphorylation of Rac1 and cdc42 proteins. It is used to measure the activation state of these small GTPases, which are involved in various cellular processes such as cytoskeleton organization, cell migration, and cell signaling.

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Lab products found in correlation

Cdc42, by Cell Signaling Technology (2 mentions) Talin, by Santa Cruz Biotechnology (1 mentions) Filamin, by Santa Cruz Biotechnology (1 mentions) Vimentin, by Santa Cruz Biotechnology (1 mentions) Phospho erk, by Cell Signaling Technology (1 mentions) U0126, by Cayman Chemical (1 mentions) Collagen 1, by Santa Cruz Biotechnology (1 mentions) Vinculin, by Santa Cruz Biotechnology (1 mentions) Rac1 2 3, by Cell Signaling Technology (1 mentions) Specific antibodies to gapdh, by Santa Cruz Biotechnology (1 mentions) Sm α actin, by Abcam (1 mentions) Proliferating cell nuclear antigen (pcna), by Santa Cruz Biotechnology (1 mentions) Y 27632, by Cayman Chemical (1 mentions) Phospho akt, by Cell Signaling Technology (1 mentions) β actin, by Abcam (1 mentions) Ly294002, by Cayman Chemical (1 mentions) Phospho gsk3α β, by Cell Signaling Technology (1 mentions) Cleaved caspase 3 asp175 5a1e, by Cell Signaling Technology (1 mentions) Phospho p70s6, by Cell Signaling Technology (1 mentions) Cyclin d1, by Cell Signaling Technology (1 mentions)

3 protocols using phospho rac1 cdc42

1

Investigating Cell Signaling Pathways

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Specific antibodies to GAPDH, vimentin, PCNA, collagen I, filamin, vinculin, and talin were purchased from Santa Cruz Biotech (Dallas, TX, USA). Polyclonal antibodies to PPARγ, α-SM-actin, and β-actin were obtained from abcam (Cambridge, UK). Polyclonal antibodies to Rho A, Rho B, Rho C, Rac1/2/3, Cdc42, phospho-Rac1/Cdc42, AKT, phospho-AKT, ERK, phospho-ERK, phospho-vimentinSer39, phospho-vimentinSer56 and phospho-vimentinSer83 were purchased from Cell Signaling (Danvers, MA, USA). U0126, Y27632, LY294002 was obtained from Cayman Chemical (Ann Arbor, Michigan, USA). DMN was purchased from Sigma-Aldrich (St. Louis, MO, USA).
Wang P.W., Wu T.H., Lin T.Y., Chen M.H., Yeh C.T, & Pan T.L. (2019). Characterization of the Roles of Vimentin in Regulating the Proliferation and Migration of HSCs during Hepatic Fibrogenesis. Cells, 8(10), 1184.
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2

Comprehensive Signaling Pathway Analysis

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Phospho-Met (Tyr1349), Phospho-Met (Tyr1003), phospho-Rac1/cdc42(Ser71), Rac1/2/3, phospho-p44/42 MAPK (Erk1/2), phospho-STAT3 (Tyr705), STAT3, Ki-67, p44/42 MAPK (Erk1/2), phospho-PDK1 (Ser241), PDK1, PTEN, PI3 kinase p110α antibody, phospho-PI3 kinase p85 (Tyr458)/p55 (Tyr199), phospho-Akt (Ser473), phospho-Akt (Thr308), phospho-mTOR (Ser2448), mTOR, phospho-GSK-3α/β (Ser21/9), GSK-3β (3D10), phospho-p70S6 kinase (T389), p70S6 kinase, phospho-4E-BP1 (Thr37/46) (236B4), 4E-BP1 (53H11), phospho-eI4FE (Ser209), eI4FE, phospho-Cyclin D1, Cyclin D1, Cyclin E1 (HE12), Cyclin A, PCNA, phospho-Rb (S807/811), Rb, Caspase-3 (8G10), Caspase-9, PARP, Bad, Cleaved caspase-3 (Asp175) (5A1E) and Phospho-Met (Tyr1234/1235) antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA). The Met kinase assay/inhibitor screening kit (# KA0055) was purchased from Abnova (Walnut, CA, USA).
Fan Y., Ding H., Kim D., Bach D.H., Hong J.Y., Xu Y, & Lee S.K. (2019). Antitumor Activity of DFX117 by Dual Inhibition of c-Met and PI3Kα in Non-Small Cell Lung Cancer. Cancers, 11(5), 627.
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3

Comprehensive Protein Analysis Protocol

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Antibodies against MITF (12590; Cell Signaling Technology, Danvers, MA, USA), GAPDH (sc-25778; Santa Cruz Biotechnology, Santa Cruz, CA, USA), p-21 (Santa Cruz Biotechnology; sc-6246), CDK-2 (Cell Signaling Technology; 2546), CDK1 (Santa Cruz Biotechnology; sc-136014), cyclin A (Santa Cruz Biotechnology; sc-271682), cyclin B1 (Santa Cruz Biotechnology; sc-245), cyclin E (Santa Cruz Biotechnology; sc-377100), phosphor-FAK (Cell Signaling Technology; 3283), FAK (Cell Signaling Technology; 3285), F-actin (ab205; Abcam, Cambridge, UK), phospho-YAP (Cell Signaling Technology; 4911), YAP (Cell Signaling Technology; 4912), lamin B (Santa Cruz Biotechnology; sc-6216), Rac1–3 (Cell Signaling Technology; 2465), Cdc42 (Cell Signaling Technology; 2466), phospho-Rac1/Cdc42 (Cell Signaling Technology; 2461), RhoA (Cell Signaling Technology; 2117 or Santa Cruz Biotechnology; sc-418) and CRIK (Santa Cruz Biotechnology; sc-390437) were used in the Western blot and immunofluorescence analyses. CN03 was purchased from Cytoskeleton (Denver, CO, USA).
Kim N., Kim S., Lee M.W., Jeon H.J., Ryu H., Kim J.M, & Lee H.J. (2021). MITF Promotes Cell Growth, Migration and Invasion in Clear Cell Renal Cell Carcinoma by Activating the RhoA/YAP Signal Pathway. Cancers, 13(12), 2920.
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