Human AML THP-1 cells were purchased from the Cell Bank of Chinese Academy of Sciences. Matrine was purchased from Tianyuan Biological Agent Plant. The Cell Counting Kit-8 (CCK-8) was purchased from Dojindo Molecular Technologies, Inc. RPMI-1640 medium, fetal bovine serum (FBS), dimethyl sulfoxide dissolving (DMSO), penicillin and streptomycin were purchased from Invitrogen; Thermo Fisher Scientific, Inc. The ELISA reader ELx800 was obtained from Bio-Tek Instruments, Inc. Inverted phase contrast microscope was obtained from Olympus Corporation. LY294002 was purchased from Cell Signaling Technology, Inc. and was dissolved in DMSO according to the manufacturer's instructions. Antibodies, including anti-PI3K, anti-phosphorylated (p)-PI3K, anti-mTOR, anti-p-mTOR, anti-Akt, anti-p-Akt and anti-β-actin were purchased from ABclonal Biotech Co., Ltd. The current study was approved by the Ethics Committee of Bengbu Medical College (Bengbu, China).
Anti p akt
Manufactured by ABclonal
Sourced in China, United States
Anti-p-Akt is a primary antibody that specifically recognizes the phosphorylated form of the Akt protein. Akt is a serine/threonine protein kinase that plays a crucial role in various cellular processes, including cell growth, proliferation, and survival. The phosphorylation of Akt is an important regulatory mechanism for its activation and function. Anti-p-Akt can be used in techniques such as Western blotting, immunoprecipitation, and immunohistochemistry to detect and quantify the levels of phosphorylated Akt in biological samples.
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Lab products found in correlation
Anti akt, by ABclonal (2 mentions)
Anti β actin, by ABclonal (2 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Penicillin, by Thermo Fisher Scientific (1 mentions)
Streptomycin, by Thermo Fisher Scientific (1 mentions)
Ly294002, by Cell Signaling Technology (1 mentions)
Inverted phase contrast microscope, by Olympus (1 mentions)
Elx800 elisa reader, by Agilent Technologies (1 mentions)
Anti pi3k, by ABclonal (1 mentions)
Anti mtor, by ABclonal (1 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions)
Crystal violet, by Biosharp (1 mentions)
Sodium new houttuyfonate, by Yuanye Bio-Technology (1 mentions)
Anti caspase 9, by ABclonal (1 mentions)
Cy3 goat anti rabbit igg h l, by ABclonal (1 mentions)
Docetaxel, by Yuanye Bio-Technology (1 mentions)
Anti gsk3β, by ABclonal (1 mentions)
Hydroxypropyl β cyclodextrin hp β cd, by Solarbio (1 mentions)
Hrp goat anti rabbit igg, by ABclonal (1 mentions)
Anti bcl 2, by ABclonal (1 mentions)
2 protocols using anti p akt
1
Matrine Inhibits AML Cell Viability
2
Efficacy of Sodium Houttuyfonate in Cancer Treatment
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The reagents used were as follows: sodium new houttuyfonate (Yuanye, Shanghai, China, CAS: 112714-99-5); docetaxel (Yuanye, Shanghai, China); hydroxypropyl-β-cyclodextrin (HP-β-CD; Solarbio, Beijing, China); N-acetyl-cysteine (NAC; Macklin, Shanghai, China); matrix adhesive (Biozellen, Frontier, NE, USA); Opti-MEM I medium (Gibco, Billings, MA, USA); and crystal violet (BioSharp, Hefei, China).
The kits used were as follows: Cell Counting Kit-8 (Hycezmbio, Wuhan, China), ROS Detection Kit (Hycezmbio, Wuhan, China), BCA Protein Quantification Kit (Hycezmbio, Wuhan, China), Apoptosis Detection Kit (Hycezmbio, Wuhan, China); and Transwell chamber (Corning, NY, USA).
According to the protocol recommended by the manufacturer, the following antibodies were used for Western blot or immunofluorescence: Anti-BAX (Wanleibio, WL01637), Anti-p-GSK3β (Wanleibio, WL03683), Anti-β-actin (ABclonal, AC038), Anti-Bcl-2 (ABclonal, A19693), Anti-cleaved PARP p25 (ABclonal, A19612), Anti-caspase-9 (ABclonal, A0281), Anti-PDK1 (ABclonal, A0834), Anti-p-PDK1 (ABclonal, AP0426), Anti-AKT (ABclonal, A20799), Anti-p-AKT (ABclonal, WLP001), Anti-GSK3β (ABclonal, A11731), Anti-MMP1 (ABclonal, A22080), HRP Goat Anti-Rabbit IgG (ABclonal, AS014), Alexa Flour 594-Goat Anti-Rabbit IgG (ABbox, AD9279), and Cy3 Goat Anti-Rabbit IgG (H + L) (ABclonal, AS007).
The kits used were as follows: Cell Counting Kit-8 (Hycezmbio, Wuhan, China), ROS Detection Kit (Hycezmbio, Wuhan, China), BCA Protein Quantification Kit (Hycezmbio, Wuhan, China), Apoptosis Detection Kit (Hycezmbio, Wuhan, China); and Transwell chamber (Corning, NY, USA).
According to the protocol recommended by the manufacturer, the following antibodies were used for Western blot or immunofluorescence: Anti-BAX (Wanleibio, WL01637), Anti-p-GSK3β (Wanleibio, WL03683), Anti-β-actin (ABclonal, AC038), Anti-Bcl-2 (ABclonal, A19693), Anti-cleaved PARP p25 (ABclonal, A19612), Anti-caspase-9 (ABclonal, A0281), Anti-PDK1 (ABclonal, A0834), Anti-p-PDK1 (ABclonal, AP0426), Anti-AKT (ABclonal, A20799), Anti-p-AKT (ABclonal, WLP001), Anti-GSK3β (ABclonal, A11731), Anti-MMP1 (ABclonal, A22080), HRP Goat Anti-Rabbit IgG (ABclonal, AS014), Alexa Flour 594-Goat Anti-Rabbit IgG (ABbox, AD9279), and Cy3 Goat Anti-Rabbit IgG (H + L) (ABclonal, AS007).
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