Mers cov antigen 725 spike protein s1

Potassium nitrate (KNO₃), gold (III) chloride solution (30 wt.% in dilute HCl), glutaraldehyde (25%), cysteamine hydrochloride, potassium ferrocyanide (K₄Fe(CN)₆), Potassium ferricyanide (K₃Fe(CN)₆), bovin serum albumin (BSA) and phosphate buffer saline (PBS) were purchased from Sigma (Ontario, Canada) (http://www.sigmaaldrich.com/canada-english.html). Antigen of HCoV (Oc43 N) and antibody for HCoV - Oc43 were purchased from Medix Biochemica (Finland) (https://www.medixbiochemica.com/en/). MERS-CoV antigen – 725 Spike protein S1 and MERS-CoV antibody were purchased from Sino Biological (Beijing, china) (https://www.sinobiological.com/). Antigen of Influenza A (N1H1) No. J8034 and Influenza B, No. J8035 were purchased from biospacific (CA, USA) (https://www.biospacific.com/).
Antigens and antibodies for both HCoV and MERS-CoV were prepared and diluted in PBS buffer, 10 mM, pH 7.4. glutaraldehyde solution was also prepared in PBS. Milli-Q water was used for the preparation of all the solutions.

The antigen for SARS-CoV-2 N protein (REC31812) and its antibody (MAB12184) were obtained from Native Antigens (Oxfordshire, UK) (https://thenativeantigencompany.com/). The MERS-CoV antigen (725 Spike protein S1) and its antibody were obtained from Sino Biological (Beijing, China) (https://www.sinobiological.com/). HCoV antigen (HK41 N) was obtained from Medix Biochemica (Finland) (https://www.medixbiochemica.com/en/). Influenza B antigen No. J8035 and Influenza A antigen (N1H1) No. J8034 were obtained from Biospacific (CA, USA) (https://www.biospacific.com/). Potassium ferricyanide (K₃[Fe(CN)₆]), potassium ferrocyanide (K₄[Fe(CN)₆]), 30 wt% gold (III) chloride solution in dilute HCl, 11-mercaptoundecanoic acid, absolute ethanol, phosphate buffer saline (PBS) and bovine serum albumin (BSA) were obtained from Sigma (Ontario, Canada) (http://www.sigmaaldrich.com/canada-english.html).
1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS) were obtained from Fisher Scientific (Ontario, Canada). The EDC/NHS solution utilized for the activation step was prepared in1X PBS buffer, pH 5.5. The solutions of the antigens and antibodies were prepared in 1X PBS buffer, pH 7.4 which was also used for the washing steps. Milli-Q water was utilized for the preparation of all the reagents throughout the study.