Graphite powder (granularity ≤30 μm) was purchased from the Sinopharm Chemical Reagent Co., Ltd., with high chemical purity. KMnO4, FeCl3·6H2O, FeSO4·7H2O, H2SO4 (98%), H2O2 (30%), and NH3·H2O of analytical grade were purchased from the Laiyang Institute of Chemical Reagents. chitosan with 85% deacetylation was obtained from the Sinopharm Chemical Reagent Co., Ltd. The chemical structures of chitin and chitosan are shown in Supplementary Fig. S5 . Glutaraldehyde (50%) was used as the cross-linking agent, which was purchased from the Tianjin Damao Chemical Reagent Co., Ltd. Potassium dichromate (K2Cr2O7) was provided by the Sinopharm Chemical Reagent Co., Ltd. All other reagents used were analytical grade without further purification. Deionized (DI) water was produced using an ultra-pure water purifier system, with a resistivity of 18.2 MΩ·cm−1.
Glutaraldehyde
Manufactured by Tianjin Damao
Sourced in China
Glutaraldehyde is a chemical compound used as a fixative and cross-linking agent in various laboratory applications. It is a colorless, oily liquid with a pungent odor. Glutaraldehyde is commonly used for the preservation and fixation of biological samples, tissue samples, and cells in microscopy, histology, and cell culture studies.
Automatically generated - may contain errors
Lab products found in correlation
Chitosan, by Sinopharm (2 mentions)
Chitin, by Sinopharm (1 mentions)
Graphite powder, by Sinopharm (1 mentions)
Potassium dichromate k2cr2o7, by Sinopharm (1 mentions)
Nano sio2, by Maclin Biochemical Technology (1 mentions)
Oleic acid, by Maclin Biochemical Technology (1 mentions)
Hydrochloric acid (hcl), by Chron Chemicals (1 mentions)
Glacial acetic acid, by Tianjin Damao (1 mentions)
Ethylene diamine tetraacetic acid (edta), by Tianjin Damao (1 mentions)
Sodium dodecyl sulfate (sds), by Solarbio (1 mentions)
Ethyl alcohol, by Tianjin Damao (1 mentions)
Nh3 h2o, by Xilong (1 mentions)
Potassium chloride (kcl), by Tianjin Kemiou Chemical Reagent (1 mentions)
Trypsin, by Solarbio (1 mentions)
Penicillin streptomycin, by Merck Group (1 mentions)
Cck 8 kit, by Dojindo Laboratories (1 mentions)
Triton x 100, by Solarbio (1 mentions)
Propidium iodide, by Beyotime (1 mentions)
Sodium chloride (nacl), by Tianjin Kemiou Chemical Reagent (1 mentions)
Calcein am, by Beyotime (1 mentions)
7 protocols using glutaraldehyde
1
Graphite-Based Composite Synthesis
2
Chitosan-Based Composite Synthesis
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Chitosan (>99%, the degree of deacetylation ≥95%), p-toluenesulfonic acid monohydrate (AR, 99%), and methanol (AR, ≥99.7%) were obtained from Chengdu Jinshan Chemical Reagent Co., Ltd. Oleic acid (AR, ≥99%) and nano-SiO2 were bought from Shanghai Macklin Biochemical Co., Ltd. Acetic acid (AR, ≥99.5%) was supplied by Chongqing Chuandong Chemical Co., Ltd. 50% of glutaraldehyde (AR, ≥99%) was purchased from Tianjin Damao Chemical Reagent Factory. Ethanol (AR, ≥99.5%) and petroleum ether (AR, 60–90°C) were obtained from Tianjin Fuyu Fine Chemical Co., Ltd. KOH (AR, ≥85.0%) was purchased from Shanghai Titan Scientific Co., Ltd.
3
Bagasse-Based Adsorption for Dye Removal
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The following chemicals were employed in this study: Glutaraldehyde (analytical grade; Tianjin Damao Chemical Reagent Factory, Tianjin, China), anhydrous sodium sulfate (analytical grade; Guangdong Chemical Reagent Engineering Technology Research and Development Center, Guangdong, China), polyvinyl alcohol 1799 (analytical grade; Chengdu Kelong Chemical Reagent Factory), zinc chloride (analytical grade; Guangdong Chemical Reagent Engineering Technology Research and Development Center), nano-titanium dioxide (Liuzhou Ruosi Nanomaterials Technology Co., Ltd., Liuzhou, China), bleached bagasse (Guigang City Gui Sugar Co., Ltd. Guigang, China), MB (analytical grade; Chengdu Kelong Chemical Reagent Factory, Chengdu, China), hydrochloric acid (analytical grade; Chengdu Kelong Chemical Co., Ltd. Chengdu, China) and sodium hydroxide (analytical grade; Guangdong Chemical Reagent Engineering Technology Research and Development Center).
4
Chitosan-Xanthan Gum Nanocomposite Synthesis
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Chitosan was provided by Bio Science & Technology Co., Ltd. (Shanghai, China). Xanthan gum was purchased from Shanghai Aladdin Biochemical Technology Co., Ltd. (Shanghai, China). CNO was kindly offered by Jiangxi Jishui Lvxiang Spice Refinery (Jishui, China). Silicon dioxide (SiO2) nanoparticles (Trade name N20) were supplied from Wacker Chemie (Munich, Germany), and the mean particle size was about 22 nm. Agar was purchased from Biosharp (Hirono, Japan). Beef extract and peptone were obtained from Guangdong Huankai Microbial Technology Co., Ltd. (Guangzhou, China). Glutaraldehyde and glacial acetic acid were offered by Tianjin Damao Chemical Reagent Factory (Tianjin, China). All reagents were used as received without any further treatments.
5
Porcine Liver and Cell Line Protocol
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Fresh porcine liver was purchased from the supermarket. L929 cell line and 4T1 cell line were donated by Zhengzhou Institute of Emerging Industrial Technology (Zhengzhou, China) and Cancer Hospital of China Medical University (Shenyang, China), respectively. NaCl, KCl, KH2PO4, and Na2HPO4·12H2O were provided by Tianjin Kemiou Chemical Reagent Co., Ltd. (Tianjin, China). Triton X-100, sodium dodecyl sulfate (SDS), and trypsin were provided by Beijing Solarbio Science & Technology Co., Ltd. (Beijing, China). NH3·H2O was provided by Xilong Scientific Co., Ltd. (Shantou, China). EDTA, glutaraldehyde, and ethyl alcohol were provided by Damao Chemical Reagent Factory (Tianjin, China). Hematoxylin and eosin (H&E) dye, Masson dye, and pepsin were provided by Beijing Coolaber Science & Technology Co., Ltd. (Beijing, China). Hoechst 33324, Calcein-AM, and propidium iodide (PI) were provided by Shanghai Beyotime Biotechnology Co., Ltd. (Shanghai, China). α-DMEM and penicillin-streptomycin were provided by Sigma-Aldrich Inc. (St. Louis, MO, USA). RPMI 1640 was provided by Procell Life Science & Technology Co., Ltd. (Wuhan, China). CCK-8 kit was provided by Dojindo Laboratories (Kumamoto, Japan).
6
Chitosan-Cyclodextrin Hydrogel Synthesis
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Reagents: chitosan (biological reagents are usually >99% pure (BR)) was obtained from Sinopharm; β-cyclodextrin (BR) and absolute ethyl alcohol were obtained from Shanghai Huishi Biochemical Reagent, Ltd.; glutaraldehyde (analytical purity reagents are usually >98% pure (AR)) and glycerol were obtained from Tianjin Damao Chemical Reagent Factory; Cos Brilliant Blue G250 was obtained from Beijing Dingguo Changsheng Biotechnology, Lt.; citric acid (AR) was obtained from Macklin; and polyethylene Glycol 400 (AR), sodium dehydration phosphate (AR), glacial acetic acid (AR), and 85% phosphoric acid (AR) were obtained from Beijing Chemical Works.
Instruments: a TD-3000 X-ray diffractometer (Dandong Tongda Technology, Co., Ltd., Dandong, China); 3K15 centrifuge (Shanghai, China, Xima Centrifuge Ltd.); NTS-4000 Constant Temperature Oscillating Water Tank (Tokyo Physical and Chemical EYELA); Nicolis5ft-IR Fourier infrared spectrometer (Waltham, MA, USA, Thermo Fisher Scientific Shier Technology); IMark microplate reader (Hercules, CA, USA, bio-rad); SL200KS optical contact angle meter (Shanghai, China, Kono Industrial Co., Ltd.); and QJ210-50N Universal Mechanical Testing Equipment were used.
Instruments: a TD-3000 X-ray diffractometer (Dandong Tongda Technology, Co., Ltd., Dandong, China); 3K15 centrifuge (Shanghai, China, Xima Centrifuge Ltd.); NTS-4000 Constant Temperature Oscillating Water Tank (Tokyo Physical and Chemical EYELA); Nicolis5ft-IR Fourier infrared spectrometer (Waltham, MA, USA, Thermo Fisher Scientific Shier Technology); IMark microplate reader (Hercules, CA, USA, bio-rad); SL200KS optical contact angle meter (Shanghai, China, Kono Industrial Co., Ltd.); and QJ210-50N Universal Mechanical Testing Equipment were used.
7
Naringinase Production from Aspergillus niger
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Aspergillus niger FFCC uv-11 was stored in our laboratory, and the naringinase fermentation broth was produced via the strain with a naringinase activity of 650.0 U mL−1. Naringin (mass fraction ≥ 98%) was purchased from Baoji Fang Sheng Biological development Co., Ltd, China. Polyethyleneimine (M.W. 10 000 Da, 99%) was obtained from Aladdin Inc. Sodium hydroxide, anhydrous ethanol, citric acid, disodium hydrogen phosphate, diethylene glycol, glutaraldehyde (50% by mass), trisodium titrate and ammonia (NH3·H2O, 25%, w/v) were purchased from Tianjin Damao Chemical Reagent Co., Ltd. Anhydrous ferric chloride (FeCl3) and ferrous sulfate heptahydrate (FeSO4·7H2O) were supplied by Sinopharm Chemical Reagent Co., Ltd.
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Aspergillus niger FFCC uv-11 was stored in our laboratory, and the naringinase fermentation broth was produced via the strain with a naringinase activity of 650.0 U mL−1. Naringin (mass fraction ≥ 98%) was purchased from Baoji Fang Sheng Biological development Co., Ltd, China. Polyethyleneimine (M.W. 10 000 Da, 99%) was obtained from Aladdin Inc. Sodium hydroxide, anhydrous ethanol, citric acid, disodium hydrogen phosphate, diethylene glycol, glutaraldehyde (50% by mass), trisodium titrate and ammonia (NH3·H2O, 25%, w/v) were purchased from Tianjin Damao Chemical Reagent Co., Ltd. Anhydrous ferric chloride (FeCl3) and ferrous sulfate heptahydrate (FeSO4·7H2O) were supplied by Sinopharm Chemical Reagent Co., Ltd.
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