Egg yolk l α phosphatidylcholine pc lecithin

Egg yolk l-α-phosphatidylcholine (PC) (lecithin) and tristearin were purchased from Sigma-Aldrich, Inc. (St. Louis, MO). The Brij52 (polyoxyethylene (2) cetyl ether) was obtained from BASF Co. (Florham Park, NJ). The sodium chloride, potassium dihydrogen orthophosphate, methanol, sodium hydroxide, magnesium chloride, chloroform, and absolute ethanol were purchased from El-Gomhouria Chemical Co. (Cairo, Egypt). The dialysis membranes (Spectra/Pore^®, cut-off 12 000–14 000) were purchased from Spectrum Laboratories Inc., Rancho Dominguez, CA). All chemicals and solvents were of analytical grade and were used as received.

The morin hydrate (MH), egg yolk L-α-phosphatidylcholine (PC) (lecithin), and triolein were purchased from Sigma-Aldrich, Inc., St. Louis, MO, USA. The Brij52 (polyoxyethylene (2) cetyl ether) was obtained from BASF Co. (Florham Park, NJ, USA). The compritol 888 (glyceryl behenate) was acquired as gift from Gattefosse (Saint-Priest, France). The sodium chloride, potassium dihydrogen orthophosphate, methanol, sodium hydroxide, magnesium chloride, chloroform, and absolute ethanol were purchased from El-Gomhouria Chemical Co., Cairo, Egypt. The dialysis membranes (Spectra/Pore^®, cut-off 12,000–14,000) were purchased from Spectrum Laboratories Inc., Rancho Dominguez, CA, USA). All chemicals and solvents were of analytical grade and were used as received. The specific ELISA kits were obtained from eBioscience Co, San Diego, CA, USA. The Vero cells (African green monkey kidney cells, Vero C1008 (Vero 76, clone E6, Vero E6)) were purchased from Viromed Laboratories (Minnetonka, MN, USA).